General Stability | Organism |
---|---|
unstable to heating, shows low activity when mantained in 50 mM Tris-HCl buffer | Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, approximately 95% inhibition, enzymatic activity is restored totally by treatment with calcium chloride or partially restored with aluminium chloride or manganese (II) chloride | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Enterobacter cloacae |
38000 | - |
1 * 38000, SDS-PAGE | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
strain M-1,intracellular and extracellular enzyme | - |
Enterobacter cloacae M-1intracellular | - |
strain M-1,intracellular and extracellular enzyme | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, cation exchange chromatography and gel filtration | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-guluronosyl linkage in alginate | endolyase | Enterobacter cloacae | ? | - |
? | |
alpha-L-guluronosyl linkage in alginate | significant activity is found not only on guluronate-guluronate linkages but also on guluronate-mannuronate linkages | Enterobacter cloacae | ? | - |
? | |
alpha-L-guluronosyl linkage in alginate | endolyase | Enterobacter cloacae M-1intracellular | ? | - |
? | |
alpha-L-guluronosyl linkage in alginate | significant activity is found not only on guluronate-guluronate linkages but also on guluronate-mannuronate linkages | Enterobacter cloacae M-1intracellular | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Enterobacter cloacae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Enterobacter cloacae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
no activity is measured after treatment at this temperature, activity decreases with increasing temperature | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Enterobacter cloacae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | stable | Enterobacter cloacae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Enterobacter cloacae | - |
- |
8.9 |