Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | dithiothreitol or 2-mercaptoethanol in concentrations between 0.1 and 6 mM have no influence on intracellular alginate lyase | Klebsiella aerogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | most of the alginate lyase activity is intracellular | Klebsiella aerogenes | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
intracellular, gel filtration | Klebsiella aerogenes |
31600 | - |
1 * 31600, intracellular, SDS-PAGE | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
type 25, intracellular and extracellular enzymes | - |
Purification (Comment) | Organism |
---|---|
partial, ammonium sulfate precipitation and ion-exchange chromatography | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-guluronosyl linkage in alginate | activity is highest on short-chain poly-guluronic acid blocks and guluronic-rich alginate, intracellular and extracellular enzymes are endo-lyases, O-acetylation and carboxyl esterification of alginate substrate inhibits intracellular enzyme action | Klebsiella aerogenes | ? | - |
? | |
additional information | requires high salt concentrations for maximal activity, no action on: laminarin, dextran, heparin, chondroitin sulfate, pullulan, yeast mannan, lichenin or porphyran, slight amylase activity with amylose and glycogen, beta-elimination mechanism, three-step reaction | Klebsiella aerogenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31600, intracellular, SDS-PAGE | Klebsiella aerogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
intracellular | Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
intracellular enzyme shows a weak residual activity of 9% | Klebsiella aerogenes |
55 | - |
intracellular enzyme shows a weak residual activity of 9% | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | intracellular enzyme | Klebsiella aerogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | sharp drop of activity below pH 6.0 and above pH 7.5 | Klebsiella aerogenes |