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Literature summary for 4.2.2.11 extracted from
- Isolation and characterization of an alginate lyase from Klebsiella aerogenes (1989), Arch. Microbiol., 152, 302-308.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | dithiothreitol or 2-mercaptoethanol in concentrations between 0.1 and 6 mM have no influence on intracellular alginate lyase | Klebsiella aerogenes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | most of the alginate lyase activity is intracellular | Klebsiella aerogenes | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Klebsiella aerogenes |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
intracellular, gel filtration | Klebsiella aerogenes |
| 31600 | - |
1 * 31600, intracellular, SDS-PAGE | Klebsiella aerogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
type 25, intracellular and extracellular enzymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, ammonium sulfate precipitation and ion-exchange chromatography | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-guluronosyl linkage in alginate | activity is highest on short-chain poly-guluronic acid blocks and guluronic-rich alginate, intracellular and extracellular enzymes are endo-lyases, O-acetylation and carboxyl esterification of alginate substrate inhibits intracellular enzyme action | Klebsiella aerogenes | ? | - |
? | |
| additional information | requires high salt concentrations for maximal activity, no action on: laminarin, dextran, heparin, chondroitin sulfate, pullulan, yeast mannan, lichenin or porphyran, slight amylase activity with amylose and glycogen, beta-elimination mechanism, three-step reaction | Klebsiella aerogenes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31600, intracellular, SDS-PAGE | Klebsiella aerogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
intracellular | Klebsiella aerogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
intracellular enzyme shows a weak residual activity of 9% | Klebsiella aerogenes |
| 55 | - |
intracellular enzyme shows a weak residual activity of 9% | Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Klebsiella aerogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | intracellular enzyme | Klebsiella aerogenes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | sharp drop of activity below pH 6.0 and above pH 7.5 | Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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