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Literature summary for 4.2.2.11 extracted from

  • Boyd, J.; Turvey, J.R.
    Isolation of poly-alpha-L-guluronate lyase from Klebsiella aerogenes (1977), Carbohydr. Res., 57, 163-171.
    View publication on PubMed
Search for more literature for 4.2.2.11

General Stability

General Stability Organism
stable to freeze-drying, retains activity for several months in this form Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 0.1 M: activation, higher concentrations cause lower activity or precipitation of alginate Klebsiella aerogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Klebsiella aerogenes
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 0.1-0.3 M, activation Klebsiella aerogenes
Mg2+ 0.05-0.1 M: activation, higher concentrations cause lower activity or precipitation of alginate Klebsiella aerogenes
Na+ 0.1-0.3 M, activation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly-alpha-L-guluronic acid Klebsiella aerogenes
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate with a smaller proportion of the homologous tetrasaccharide ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
 type 25, intracellular and extracellular enzymes
-

Purification (Commentary)

Purification (Comment) Organism
partial, ammonium sulfate precipitation and ion-exchange chromatography Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-guluronosyl linkage in alginate
-
 Klebsiella aerogenes ?
-
 ?
additional information requires high salt concentrations for maximal activity, no action on: laminarin, dextran, heparin, chondroitin sulfate, pullulan, yeast mannan, lichenin or porphyran, slight amylase activity with amylose and glycogen, beta-elimination mechanism, three-step reaction Klebsiella aerogenes ?
-
 ?
poly-alpha-L-guluronic acid
-
 Klebsiella aerogenes ?
-
 ?
poly-alpha-L-guluronic acid
-
 Klebsiella aerogenes unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate with a smaller proportion of the homologous tetrasaccharide ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Klebsiella aerogenes