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Literature summary for 4.2.2.10 extracted from

  • Perez-Fuentes, C.; Cristina Ravanal, M.; Eyzaguirre, J.
    Heterologous expression of a Penicillium purpurogenum pectin lyase in Pichia pastoris and its characterization (2014), Fungal Biol., 118, 507-515.
    View publication on PubMed
Search for more literature for 4.2.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Talaromyces purpureogenus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Talaromyces purpureogenus

Organism

Organism UniProt Comment Textmining
Talaromyces purpureogenus R9XVW6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin the activity increases with the degree of esterification, best substrate shows a degree of esterification of 85% Talaromyces purpureogenus ?
-
 ?
additional information enzyme is active on highly esterified pectin, and decreases 40% the viscosity of pectin with a degree of esterification of 85%. The enzyme shows no activity on polygalacturonic acid and pectin from citrus fruit which is 8% esterified Talaromyces purpureogenus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Talaromyces purpureogenus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Talaromyces purpureogenus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 3 h, stable up to Talaromyces purpureogenus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Talaromyces purpureogenus