Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.10 extracted from

  • Semenova, M.V.; Grishutin, S.G.; Gusakov, A.V.; Okunev, O.N.; Sinitsyn, A.P.
    Isolation and properties of pectinases from the fungus Aspergillus japonicus (2003), Biochemistry (Moscow), 68, 559-569.
    View publication on PubMed
Search for more literature for 4.2.2.10

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values of pectin substrates with different degree of methylation Aspergillus japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Aspergillus japonicus

Organism

Organism UniProt Comment Textmining
Aspergillus japonicus
-
 mutant strain
-
Aspergillus japonicus mutant
-
 mutant strain
-

Purification (Commentary)

Purification (Comment) Organism
263fold Aspergillus japonicus

Reaction

Reaction Comment Organism Reaction ID
[6-O-methyl-alpha-D-GalpA]n = [6-O-methyl-alpha-D-GalpA]m + 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1 acts antagonistic to pectinesterase, EC 3.1.1.11 Aspergillus japonicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with polygalacturonic acid, acts antagonistic to pectinesterase, EC 3.1.1.11 Aspergillus japonicus ?
-
 ?
additional information no activity with polygalacturonic acid, acts antagonistic to pectinesterase, EC 3.1.1.11 Aspergillus japonicus mutant ?
-
 ?
pectin citrus pectin, activity increases with increasing degree of methylation of the substrate Aspergillus japonicus DELTA4,5-unsaturated oligosaccharides
-
 ?
pectin citrus pectin, activity increases with increasing degree of methylation of the substrate Aspergillus japonicus mutant DELTA4,5-unsaturated oligosaccharides
-
 ?
pectin 26% methylated
-
 Aspergillus japonicus ?
-
 ?
pectin 26% methylated
-
 Aspergillus japonicus mutant ?
-
 ?
pectin 65% methylated
-
 Aspergillus japonicus ?
-
 ?
pectin 65% methylated
-
 Aspergillus japonicus mutant ?
-
 ?
pectin 89% methylated
-
 Aspergillus japonicus ?
-
 ?
pectin 89% methylated
-
 Aspergillus japonicus mutant ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Aspergillus japonicus

Synonyms

Synonyms Comment Organism
pectin lyase
-
 Aspergillus japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Aspergillus japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 3 h, completely stable Aspergillus japonicus
50
-
 3 h, loss of 10% activity Aspergillus japonicus
60
-
 3 h, loss of 55% activity Aspergillus japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 pectin 26% methylated pH 5.0, 40°C Aspergillus japonicus
77
-
 pectin 65% methylated pH 5.0, 40°C Aspergillus japonicus
86
-
 pectin 89% methylated pH 5.0, 40°C Aspergillus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Aspergillus japonicus

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 50% of maximal activity at pH 4.0 and pH 6.5 Aspergillus japonicus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus japonicus isoelectric focusing
-
 4.2