Crystallization (Comment) | Organism |
---|---|
enzyme-substrate complexes, wild-type enzyme and mutants H399A, and Y408F, each 5 mg/ml in 10 mM Tris-HCl, pH 7.4, 2 mM EDTA, hanging drop vapour diffusion method, equal volumes of protein, 50 mM chondroitin in 10 mM Tris-HCl solution, and reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.56 A resolution | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan | Streptococcus pneumoniae | enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q54873 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | active site structure and substrate binding of wild-type and mutant enzymes with chondroitin, mode of action | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chondroitin | non-progressive mode | Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-galactosamine + ? | unsaturated disaccharide units | ? | |
chondroitin sulfate | non-progressive mode, no activity with chondroitin 4-sulfated at the nonreducing end, no activity with substrate sulfated at position 2 | Streptococcus pneumoniae | ? | - |
? | |
hyaluronan | enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue | Streptococcus pneumoniae | ? | - |
? | |
hyaluronan | progressive mode, preferred substrate | Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide units | ? | |
additional information | substrate specificity | Streptococcus pneumoniae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HL | - |
Streptococcus pneumoniae |