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Literature summary for 4.2.2.1 extracted from

  • Rigden, D.J.; Jedrzejas, M.J.
    Structures of Streptococcus pneumoniae hyaluronate lyase in complex with chondroitin and chondroitin sulfate disaccharides. Insights into specificity and mechanism of action (2003), J. Biol. Chem., 278, 50596-50606.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme-substrate complexes, wild-type enzyme and mutants H399A, and Y408F, each 5 mg/ml in 10 mM Tris-HCl, pH 7.4, 2 mM EDTA, hanging drop vapour diffusion method, equal volumes of protein, 50 mM chondroitin in 10 mM Tris-HCl solution, and reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.56 A resolution Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyaluronan Streptococcus pneumoniae enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue ?
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?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q54873
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-

Reaction

Reaction Comment Organism Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate active site structure and substrate binding of wild-type and mutant enzymes with chondroitin, mode of action Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin non-progressive mode Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-galactosamine + ? unsaturated disaccharide units ?
chondroitin sulfate non-progressive mode, no activity with chondroitin 4-sulfated at the nonreducing end, no activity with substrate sulfated at position 2 Streptococcus pneumoniae ?
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?
hyaluronan enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue Streptococcus pneumoniae ?
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?
hyaluronan progressive mode, preferred substrate Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine unsaturated disaccharide units ?
additional information substrate specificity Streptococcus pneumoniae ?
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?

Synonyms

Synonyms Comment Organism
HL
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Streptococcus pneumoniae