Cloned (Comment) | Organism |
---|---|
gene CYP74Q1, DNA and amino acid sequence determination and analysis, sequence comparisons | Ranunculus acris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0431 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienate | pH 7.5, 25°C | Ranunculus acris | |
0.0976 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C | Ranunculus acris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Ranunculus acris | - |
(9Z,11E)-12-[(1'Z)-hexenyloxy]-9,11-dodecadienoic acid | i.e. (omega5Z)-etherolenic acid | ? | |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate | Ranunculus acris | highly preferred substrate | (9Z,11E)-12-[(1'Z,3'Z)-hexadienyloxy]-9,11-dodecadienoate + H2O | i.e. etherolenic acid | ? | |
additional information | Ranunculus acris | preferred substrates of the enzyme are the 13-hydroperoxides of alpha-linolenic and linoleic acids, which are converted to the divinyl ether oxylipins (omega5Z)-etherolenic acid, (9Z,11E)-12-[(1?Z,3?Z)-hexadienyloxy]-9,11-dodecadienoic acid, and (omega5Z)-etheroleic acid, (9Z,11E)-12-[(1?Z)-hexenyloxy]-9,11-dodecadienoic acid, respectively, as revealed by the data of mass spectrometry, NMR and UV spectroscopy | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ranunculus acris | - |
two CYP74Q1 isoforms, gene CYP74Q1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Ranunculus acris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Ranunculus acris | (9Z,11E)-12-[(1'Z)-hexenyloxy]-9,11-dodecadienoic acid | i.e. (omega5Z)-etherolenic acid | ? | |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate | highly preferred substrate | Ranunculus acris | (9Z,11E)-12-[(1'Z,3'Z)-hexadienyloxy]-9,11-dodecadienoate + H2O | i.e. etherolenic acid | ? | |
additional information | preferred substrates of the enzyme are the 13-hydroperoxides of alpha-linolenic and linoleic acids, which are converted to the divinyl ether oxylipins (omega5Z)-etherolenic acid, (9Z,11E)-12-[(1?Z,3?Z)-hexadienyloxy]-9,11-dodecadienoic acid, and (omega5Z)-etheroleic acid, (9Z,11E)-12-[(1?Z)-hexenyloxy]-9,11-dodecadienoic acid, respectively, as revealed by the data of mass spectrometry, NMR and UV spectroscopy | Ranunculus acris | ? | - |
? | |
additional information | no activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid and (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid, high substrate specificity, overview. Product identification by GC-MS analyses | Ranunculus acris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP74Q1 | - |
Ranunculus acris |
DES | - |
Ranunculus acris |
divinyl ether synthase | - |
Ranunculus acris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ranunculus acris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67.7 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C | Ranunculus acris | |
134 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienate | pH 7.5, 25°C | Ranunculus acris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Ranunculus acris |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the CYP74 family, phylogenetic tree | Ranunculus acris |
physiological function | enzymes of the CYP74 family, including the divinyl ether synthase, play important roles in plant cell signalling and defence | Ranunculus acris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.5, 25°C | Ranunculus acris | |
0.0031 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienate | pH 7.5, 25°C | Ranunculus acris |