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Literature summary for 4.2.1.B27 extracted from

  • Yu, X.; Pérez, B.; Zhang, Z.; Gao, R.; Guo, Z.
    Mining catalytic promiscuity from thermophilic archaea: an acyl-peptide releasing enzyme from Sulfolobus tokodaii (ST0779) for nitroaldol reactions (2016), Green Chem., 18, 2753-2761.
No PubMed abstract available
Search for more literature for 4.2.1.B27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
 4-nitrobenzaldehyde 40°C, pH not specified in the publication Sulfurisphaera tokodaii
0.72
-
 4-nitrobenzaldehyde 30°C, pH not specified in the publication Sulfurisphaera tokodaii

Organic Solvent Stability

Organic Solvent Comment Organism
additional information reactions performed in n-propanol, dimethylsulfoxide and N,N-dimethyl formamide only give traces of products. There is no evident correlation between solvent polarity and catlytic efficiency Sulfurisphaera tokodaii
tert-butyl methyl ether and tetrahydrofuran, give the highest product yields Sulfurisphaera tokodaii
tetrahydrofuran and tert-butyl methyl ether, give the highest product yields Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q973W9 acylaminoacyl-peptidase, EC 3.4.19.1, additionally catalyzes nitroaldol (Henry) reaction
-
Sulfurisphaera tokodaii DSM 16993 Q973W9 acylaminoacyl-peptidase, EC 3.4.19.1, additionally catalyzes nitroaldol (Henry) reaction
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii 2-nitro-1-(2-nitrophenyl)ethanol 90% yield, 93% enantiomeric excess ?
2-nitrobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii DSM 16993 2-nitro-1-(2-nitrophenyl)ethanol 90% yield, 93% enantiomeric excess ?
3-nitrobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii 2-nitro-1-(3-nitrophenyl)ethanol 89% yield, 99% enantiomeric excess ?
3-nitrobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii DSM 16993 2-nitro-1-(3-nitrophenyl)ethanol 89% yield, 99% enantiomeric excess ?
4-cyanobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii 2-nitro-1-(4-cyanophenyl)ethanol 92% yield, 99% enantiomeric excess ?
4-cyanobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii DSM 16993 2-nitro-1-(4-cyanophenyl)ethanol 92% yield, 99% enantiomeric excess ?
4-nitrobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii 2-nitro-1-(4-nitrophenyl)ethanol 92% yield, 93% enantiomeric excess ?
4-nitrobenzaldehyde + nitromethane
-
 Sulfurisphaera tokodaii DSM 16993 2-nitro-1-(4-nitrophenyl)ethanol 92% yield, 93% enantiomeric excess ?

Synonyms

Synonyms Comment Organism
STK_07790
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 4-nitrobenzaldehyde 30°C, pH not specified in the publication Sulfurisphaera tokodaii
0.45
-
 4-nitrobenzaldehyde 40°C, pH not specified in the publication Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.485
-
 4-nitrobenzaldehyde 30°C, pH not specified in the publication Sulfurisphaera tokodaii
0.731
-
 4-nitrobenzaldehyde 40°C, pH not specified in the publication Sulfurisphaera tokodaii