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Literature summary for 4.2.1.93 extracted from

  • Colinas, M.; Shaw, H.; Loubery, S.; Kaufmann, M.; Moulin, M.; Fitzpatrick, T.
    A pathway for repair of NAD(P)H in plants (2014), J. Biol. Chem., 289, 14692-14706.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene At5g19150, sequence comparisons, recombinant expression of C-terminally His6-tagged in Escherichia coli strain BL21(DE3), expression of YFP-tagged enzyme in Arabidopsis thaliana cells Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-
mitochondrion
-
Arabidopsis thaliana 5739
-
additional information the enzyme is predominantly (if not exclusively) found in chloroplasts and mitochondria Arabidopsis thaliana
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35701
-
4 * 35701, His-tagged enzyme, mass spectrometry, and gel filtration coupled to static light scattering Arabidopsis thaliana
132700
-
about, gel filtration coupled to static light scattering Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana
-
ADP + phosphate + NADH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Col-0
-
ADP + phosphate + NADH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana
-
ADP + phosphate + NADPH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Col-0
-
ADP + phosphate + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q94AF2 gene At5g19150
-
Arabidopsis thaliana Col-0 Q94AF2 gene At5g19150
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf rosette and cauline leaves, weak expression Arabidopsis thaliana
-
additional information tissue expression profile, overview Arabidopsis thaliana
-
root weak expression Arabidopsis thaliana
-
seed dry, main expression Arabidopsis thaliana
-
seedling weak expression Arabidopsis thaliana
-
silique weak expression Arabidopsis thaliana
-
stem weak expression Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
Arabidopsis thaliana ADP + phosphate + NADH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana ADP + phosphate + NADH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
Arabidopsis thaliana Col-0 ADP + phosphate + NADH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Col-0 ADP + phosphate + NADH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
Arabidopsis thaliana ADP + phosphate + NADPH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana ADP + phosphate + NADPH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
Arabidopsis thaliana Col-0 ADP + phosphate + NADPH
-
?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Col-0 ADP + phosphate + NADPH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 35701, His-tagged enzyme, mass spectrometry, and gel filtration coupled to static light scattering Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
ATP-dependent NNRD
-
Arabidopsis thaliana
NAD(P)X dehydratase
-
Arabidopsis thaliana
NNRD
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the stereospecific dehydratase is involved in a potential NAD(P)H repair pathway in plants. Dehydratase NNRD and an epimerase (NNRE), fused to a vitamin B6 salvage enzyme, act concomitantly to restore NAD(P)HX to NAD(P)H, but the proteins do not physically interact Arabidopsis thaliana
additional information homology models of the three-dimensional structures of NAD(P)X dehydratase and NAD(P)X epimerase Arabidopsis thaliana
physiological function the enzyme is involved in the NAD(P)H repair pathway but is dispensable for growth and development under standard conditions Arabidopsis thaliana