Cloned (Comment) | Organism |
---|---|
gene At5g19150, sequence comparisons, recombinant expression of C-terminally His6-tagged in Escherichia coli strain BL21(DE3), expression of YFP-tagged enzyme in Arabidopsis thaliana cells | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
additional information | the enzyme is predominantly (if not exclusively) found in chloroplasts and mitochondria | Arabidopsis thaliana | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35701 | - |
4 * 35701, His-tagged enzyme, mass spectrometry, and gel filtration coupled to static light scattering | Arabidopsis thaliana |
132700 | - |
about, gel filtration coupled to static light scattering | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana | - |
ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana Col-0 | - |
ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana | - |
ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana Col-0 | - |
ADP + phosphate + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q94AF2 | gene At5g19150 | - |
Arabidopsis thaliana Col-0 | Q94AF2 | gene At5g19150 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | rosette and cauline leaves, weak expression | Arabidopsis thaliana | - |
additional information | tissue expression profile, overview | Arabidopsis thaliana | - |
root | weak expression | Arabidopsis thaliana | - |
seed | dry, main expression | Arabidopsis thaliana | - |
seedling | weak expression | Arabidopsis thaliana | - |
silique | weak expression | Arabidopsis thaliana | - |
stem | weak expression | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana Col-0 | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana Col-0 | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana | ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana | ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana Col-0 | ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana Col-0 | ADP + phosphate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35701, His-tagged enzyme, mass spectrometry, and gel filtration coupled to static light scattering | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent NNRD | - |
Arabidopsis thaliana |
NAD(P)X dehydratase | - |
Arabidopsis thaliana |
NNRD | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the stereospecific dehydratase is involved in a potential NAD(P)H repair pathway in plants. Dehydratase NNRD and an epimerase (NNRE), fused to a vitamin B6 salvage enzyme, act concomitantly to restore NAD(P)HX to NAD(P)H, but the proteins do not physically interact | Arabidopsis thaliana |
additional information | homology models of the three-dimensional structures of NAD(P)X dehydratase and NAD(P)X epimerase | Arabidopsis thaliana |
physiological function | the enzyme is involved in the NAD(P)H repair pathway but is dispensable for growth and development under standard conditions | Arabidopsis thaliana |