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Literature summary for 4.2.1.92 extracted from

  • Ono, E.; Handa, T.; Koeduka, T.; Toyonaga, H.; Tawfik, M.M.; Shiraishi, A.; Murata, J.; Matsui, K.
    CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis) (2016), Plant Physiol. Biochem., 98, 112-118 .
    View publication on PubMed
Search for more literature for 4.2.1.92

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP74B24, three HPL-related genes from Camellia sinensis are identified via RNA-sequencing in silico, gene CYP74B24 encodes a functional tea HPL enzyme, recombinant expression of CYP74B24 protein in Escherichia coli Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Camellia sinensis
-
 hexanal + 12-oxo-9-dodecenoic acid
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Camellia sinensis
-
 (Z)-3-hexenal + 12-oxo-9-dodecenoic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis G4VV60
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf constitutive expression of CYP74B24 gene in intact tea leaves Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Camellia sinensis hexanal + 12-oxo-9-dodecenoic acid
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Camellia sinensis (Z)-3-hexenal + 12-oxo-9-dodecenoic acid
-
 ?
additional information the recombinant enzyme (encoded by gene CYP74B24) produces (Z)-3-hexenal from 13-HPOT with the optimal pH 6.0 in vitro Camellia sinensis ?
-
 ?

Synonyms

Synonyms Comment Organism
13HPL
-
 Camellia sinensis
CYP74B24
-
 Camellia sinensis
hydroperoxide lyase
-
 Camellia sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme Camellia sinensis

Expression

Organism Comment Expression
Camellia sinensis after mechanical wounding, the enzyme activity and amounts of volatiles increase significantly. Formation of (Z)-3-hexenal is rapidly but transiently enhanced after wounding, and only a trace amount is detected after 10 min of wounding. 4-Oxo-(E)-2-hexenal, which was an oxidation product of (Z)-3-hexenal, is also formed quickly after wounding up

General Information

General Information Comment Organism
metabolism HPL-mediated GLV metabolism in tea plant, overview Camellia sinensis
physiological function green leaf volatiles (GLVs) are C6-aliphatic aldehydes/alcohols/acetates, and biosynthesized from the central precursor fatty acid 13-hydroperoxides by 13-hydroperoxide lyases (HPLs) in various plant species. While GLVs have been implicated as defense compounds in plants, GLVs give characteristic grassy note to a bouquet of aroma in green tea, which is manufactured from young leaves of Camellia sinensis. Gene CYP74B24 encodes tea HPL enzyme. Constitutive expression of CYP74B24 gene in intact tea leaves might account for low but substantial and constitutive formation of a subset of GLVs, some of which are stored as glycosides Camellia sinensis