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Literature summary for 4.2.1.92 extracted from

  • Teder, T.; Boeglin, W.E.; Schneider, C.; Brash, A.R.
    A fungal catalase reacts selectively with the 13S fatty acid hydroperoxide products of the adjacent lipoxygenase gene and exhibits 13S-hydroperoxide-dependent peroxidase activity (2017), Biochim. Biophys. Acta, 1862, 706-715 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.92

Cloned(Commentary)

Cloned (Comment) Organism
gene FG02216.1, transient transfection in HeLa cells using VTF-7, a recombinant vaccinia virus containing the T7 RNA polymerase gene, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Fusarium graminearum

Inhibitors

Inhibitors Comment Organism Structure
(13S)-hydroperoxyoctadecadienoic acid Fg-cat is inactivated in the course of the transformation of 13S-hydroperoxyoctadecadienoic acid and reaction is reinitiated by further addition of enzyme, product inhibition Fusarium graminearum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(13S)-hydroperoxy-(9Z,11E)-octadecadienoic acid Fusarium graminearum
-
 (12,13S)-oxido-(9Z,11)-octadecadienoic acid + H2O
-
 ?
(13S)-hydroperoxy-(9Z,11E)-octadecadienoic acid Fusarium graminearum PH-1
-
 (12,13S)-oxido-(9Z,11)-octadecadienoic acid + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium graminearum I1REW2 i.e. Gibberella zeae
-
Fusarium graminearum PH-1 I1REW2 i.e. Gibberella zeae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13S)-hydroperoxy-(9Z,11E)-octadecadienoic acid
-
 Fusarium graminearum (12,13S)-oxido-(9Z,11)-octadecadienoic acid + H2O
-
 ?
(13S)-hydroperoxy-(9Z,11E)-octadecadienoic acid
-
 Fusarium graminearum PH-1 (12,13S)-oxido-(9Z,11)-octadecadienoic acid + H2O
-
 ?
additional information mechanism of formation of fatty acid diepoxy ketone, overview. No reaction with 9R- and 9S-hydroperoxy-9,11-octadecadienoates. Peroxidase assay monitoring the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS), enzyme Fg-cat exhibits robust activity (kcat 550 s/1) using the 13S-hydroperoxy-C18 fatty acids as the oxidizing co-substrate Fusarium graminearum ?
-
 ?
additional information mechanism of formation of fatty acid diepoxy ketone, overview. No reaction with 9R- and 9S-hydroperoxy-9,11-octadecadienoates. Peroxidase assay monitoring the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS), enzyme Fg-cat exhibits robust activity (kcat 550 s/1) using the 13S-hydroperoxy-C18 fatty acids as the oxidizing co-substrate Fusarium graminearum PH-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
13S-hydroperoxide-dependent peroxidase
-
 Fusarium graminearum
fatty acid hydroperoxide-metabolizing enzyme
-
 Fusarium graminearum
Fg-cat
-
 Fusarium graminearum
FG02216.1
-
 Fusarium graminearum
FGSG_02217
-
 Fusarium graminearum
LOX
-
 Fusarium graminearum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Fusarium graminearum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Fusarium graminearum

Cofactor

Cofactor Comment Organism Structure
heme
-
 Fusarium graminearum

General Information

General Information Comment Organism
additional information the enzyme has the distal heme signature sequence of Thr-His Fusarium graminearum