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Literature summary for 4.2.1.92 extracted from

  • Bruehlmann, F.; Bosijokovic, B.; Ullmann, C.; Auffray, P.; Fourage, L.; Wahler, D.
    Directed evolution of a 13-hydroperoxide lyase (CYP74B) for improved process performance (2013), J. Biotechnol., 163, 339-345.
    View publication on PubMed
Search for more literature for 4.2.1.92

Cloned(Commentary)

Cloned (Comment) Organism
gene 13-hpl, sequence comparisons, functional expression in Escherichia coli partly in inculsion bodies, fusion of the maltose binding protein malE to the 5'-truncated 13-hpl gene improves functional expression to some extent Psidium guajava

Protein Variants

Protein Variants Comment Organism
additional information gene 13-hpl is used for random mutagenesis via gene shuffling using template based recombination of restriction fragments and in vitro ligations (L-shuffling) with DNA sequences from melon, Nicotiana attenuata (GenBank accession No. AJ414400), Citrus sinensis (GenBank accession No. AY242385), and Malus domestica and a guava hpl gene containing the 5'DELTA93 bp deletion. Mutant sequence analysis, overview. The mutant variants show highly increased trunover as well as reduced mechanism based suicide inactivation compared to the wild-type Psidium guajava

Inhibitors

Inhibitors Comment Organism Structure
additional information mechanism based suicide inactivation is a common feature of hydroperoxide lyases and many other enzymes of the P450 family Psidium guajava

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme enzyme Psidium guajava

Organism

Organism UniProt Comment Textmining
Psidium guajava
-
 gene 13-hpl
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Psidium guajava ?
-
 ?
13-hydroperoxy-9,11-octadecadienoic acid
-
 Psidium guajava ?
-
 ?
additional information in case of hydroperoxide lyases the homolytic isomerization of fatty acid hydroperoxides leads to a short-lived hemiacetal, which spontaneously disintegrates into (Z)-3-hexenal and (9Z)-12-oxo-9-dodecenoic acid Psidium guajava ?
-
 ?

Synonyms

Synonyms Comment Organism
13-HPL
-
 Psidium guajava
13-hydroperoxide lyase
-
 Psidium guajava
CYP74B
-
 Psidium guajava

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Psidium guajava

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Psidium guajava

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 heme containing Psidium guajava

General Information

General Information Comment Organism
evolution allene oxide synthases, like hydroperoxide lyases and divinyl ether synthases belong to the CYP74 family of cytochrome P450 enzymes. These enzymes rearrange fatty acid hydroperoxides differently, sharing an epoxyallylic radical as a common intermediate Psidium guajava