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Literature summary for 4.2.1.92 extracted from

  • Hoffmann, I.; Jerneren, F.; Oliw, E.H.
    Expression of fusion proteins of Aspergillus terreus reveals a novel allene oxide synthase (2013), J. Biol. Chem., 288, 11459-11469.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.92

Cloned(Commentary)

Cloned (Comment) Organism
gene ATEG_02036, sequence comparisons with other enzymes, cloning and functional expression of the enzyme with V5 epitope and His6-tag from plasmid pIZ/V5-His in Spodoptera frugiperda Sf9 cells and in Escherichia coli Aspergillus terreus

Protein Variants

Protein Variants Comment Organism
C1073S site-directed mutagenesis, the substitution in the P450 domain abolishes allene oxide synthase activity Aspergillus terreus
N964D site-directed mutagenesis, the mutation markedly reduces allene oxide synthase activity Aspergillus terreus
N964V site-directed mutagenesis, the mutation markedly reduces allene oxide synthase activity Aspergillus terreus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Aspergillus terreus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133000
-
 recombinant C-terminally His6-tagged, V5-tagged enzyme Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Aspergillus terreus
-
 (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Aspergillus terreus A1156
-
 (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?

Organism

Organism UniProt Comment Textmining
Aspergillus terreus Q0CW98 gene ATEG_02036; gene ATEG_02036
-
Aspergillus terreus A1156 Q0CW98 gene ATEG_02036; gene ATEG_02036
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus terreus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Aspergillus terreus (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Aspergillus terreus A1156 (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?
additional information Asn964 may facilitate homolytic cleavage of the dioxygen bond of (9R)-hydroperoxy-10(E),12(Z)-octadecadienoic acid with formation of compound II. No activity with 18:2n-6 Aspergillus terreus ?
-
 ?
additional information Asn964 may facilitate homolytic cleavage of the dioxygen bond of (9R)-hydroperoxy-10(E),12(Z)-octadecadienoic acid with formation of compound II. No activity with 18:2n-6 Aspergillus terreus A1156 ?
-
 ?

Synonyms

Synonyms Comment Organism
9R-DOX-AOS
-
 Aspergillus terreus
allene oxide synthase
-
 Aspergillus terreus
AOS
-
 Aspergillus terreus
KC525886
-
 Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 6003B Aspergillus terreus

General Information

General Information Comment Organism
evolution the fungal allene oxide synthases belongs to the CYP74 family Aspergillus terreus