Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | AOS is a non-classical cytochrome P450, which, like other CYP74 enzymes, does not require molecular oxygen and NADPH-dependent P450 reductase | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
imidazole | imidazole may coordinate to ferric heme iron, triggering a heme-iron transition from high spin state to low spin state | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0069 | - |
(9S)-hydroperoxyoctadecadienoic acid | pH 7.5, 22°C | Oryza sativa | |
0.0086 | - |
(9S)-hydroperoxyoctadecatrienoic acid | pH 7.5, 22°C | Oryza sativa | |
0.0123 | - |
(13S)-hydroperoxyoctadecadienoic acid | pH 7.5, 22°C | Oryza sativa | |
0.0125 | - |
(13S)-hydroperoxyoctadecatrienoic acid | pH 7.5, 22°C | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | ferric heme iron | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Oryza sativa | cleavage of 9-hydroperoxy fatty acids in vivo, AOS enzymes also convert hydroperoxy fatty acids to aldehydes via an HPL-like activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(13S)-hydroperoxyoctadecadienoic acid | - |
Oryza sativa | 13-hydroxy-12-oxo-9(Z)-octadecenoic acid + 12-oxo-10-phytoenoic acid | 13-hydroxy-12-oxo-(9Z)-octadecenoic acid is the major product | ? | |
(13S)-hydroperoxyoctadecatrienoic acid | - |
Oryza sativa | 13-hydroxy-12-oxo-(9Z,15Z)-octadecadienoic acid + 12-oxo-(10Z,15Z)-phytodienoic acid | OsAOS1 produces a 1:1 racemic mixture of cis-(+)/cis-(-)-12-oxo-(10Z,15Z)-phytodienoic acid via non-enzymatic cyclization of 12,13-epoxy-9,11,15-octadecatrienoic acid, an unstable allene oxide | ? | |
(9S)-hydroperoxyoctadecadienoic acid | - |
Oryza sativa | ? | - |
? | |
(9S)-hydroperoxyoctadecatrienoic acid | - |
Oryza sativa | ? | - |
? | |
additional information | cleavage of 9-hydroperoxy fatty acids in vivo, AOS enzymes also convert hydroperoxy fatty acids to aldehydes via an HPL-like activity | Oryza sativa | ? | - |
? | |
additional information | allene oxide synthase converts fatty acid hydroperoxides into unstable fatty acid epoxides (allene oxides). OsAOS1 exhibits dual positional substrate specificity and prefers 13-positional isomeric to 9-positional isomeric substrates in vitro. OsAOS1 converts (9S)-hydroperoxyoctadecadienoic acid or (9S)-hydroperoxyoctadecatrienoic acid into the corresponding unstable allene oxides, which are non-enzymatically transformed into a 9alpha-ketol as the major product, and 13gamma-ketols and cyclopentenone derivatives as minor products | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
allene oxide synthase-1 | - |
Oryza sativa |
CYP74 | - |
Oryza sativa |
OsAOS1 | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.5 | - |
(9S)-hydroperoxyoctadecatrienoic acid | pH 7.5, 22°C | Oryza sativa | |
44.9 | - |
(9S)-hydroperoxyoctadecadienoic acid | pH 7.5, 22°C | Oryza sativa | |
494.5 | - |
(13S)-hydroperoxyoctadecadienoic acid | pH 7.5, 22°C | Oryza sativa | |
537.8 | - |
(13S)-hydroperoxyoctadecatrienoic acid | pH 7.5, 22°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
evolution | AOS is a non-classical cytochrome P450, which, like other CYP74 enzymes, does not require molecular oxygen and NADPH-dependent P450 reductase | Oryza sativa |
additional information | putative type II ligand-induced spin state transition in OsAOS1 | Oryza sativa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2000 | - |
(9S)-hydroperoxyoctadecatrienoic acid | pH 7.5, 22°C | Oryza sativa | |
6500 | - |
(9S)-hydroperoxyoctadecadienoic acid | pH 7.5, 22°C | Oryza sativa | |
39600 | - |
(13S)-hydroperoxyoctadecadienoic acid | pH 7.5, 22°C | Oryza sativa | |
43700 | - |
(13S)-hydroperoxyoctadecatrienoic acid | pH 7.5, 22°C | Oryza sativa |