Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.92 extracted from

  • Brash, A.
    Mechanistic aspects of CYP74 allene oxide synthases and related cytochrome P450 enzymes (2009), Phytochemistry, 70, 1522-1531.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information exhibits weak activity prior to activation (in vitro), can be massively activated with detergent Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
-
Parthenium argentatum

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Arabidopsis thaliana
is a typical P450, the loop forms a compact beta-turn and incorporates the most typical P450 consensus sequence, FXXG, followed by three residues (XRX) followed by CXG encompassing the cysteine. CYP74 heme is strongly hold in place than usual via ionic interactions with its proprionate groups Parthenium argentatum

Metals/Ions

Metals/Ions Comment Organism Structure
heme CYP74 heme is strongly hold in place than usual via ionic interactions with its proprionate groups Parthenium argentatum
Iron
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
Parthenium argentatum

Organism

Organism UniProt Comment Textmining
Acaryochloris marina
-
-
-
Arabidopsis thaliana
-
-
-
Linum usitatissimum
-
seed
-
Parthenium argentatum
-
-
-
Solanum lycopersicum
-
-
-
Solanum tuberosum
-
-
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Linum usitatissimum
-
Parthenium argentatum

Source Tissue

Source Tissue Comment Organism Textmining
additional information CYP74A2 is most abundant in rubber, accounting for about 50% of the protein content Parthenium argentatum
-
seed
-
Linum usitatissimum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CYP74 enzymes use their fatty acid hydroperoxide substrate to activate the enzyme and substrate in one and the same step Linum usitatissimum ?
-
?

Synonyms

Synonyms Comment Organism
allene oxide synthase
-
Zea mays
allene oxide synthase
-
Solanum tuberosum
allene oxide synthase
-
Arabidopsis thaliana
allene oxide synthase
-
Solanum lycopersicum
allene oxide synthase
-
Linum usitatissimum
allene oxide synthase
-
Parthenium argentatum
allene oxide synthase
-
Acaryochloris marina
AOS
-
Zea mays
AOS
-
Solanum tuberosum
AOS
-
Arabidopsis thaliana
AOS
-
Solanum lycopersicum
AOS
-
Linum usitatissimum
AOS
-
Parthenium argentatum
AOS
-
Acaryochloris marina
CYP74
-
Linum usitatissimum
CYP741
-
Arabidopsis thaliana
CYP742
-
Parthenium argentatum
CYP74A
-
Zea mays
CYP74c
-
Solanum tuberosum
CYP74c
-
Solanum lycopersicum

General Information

General Information Comment Organism
metabolism role for Phe137 in catalysis, mutation to Leu switches the product outcome from AOS to hydroperoxide lyase Arabidopsis thaliana
physiological function is a cytochrome P450 Linum usitatissimum
physiological function is a potential rubber transferase, the enzyme polymerizes thousands of isoprenes into molecules of rubber. Plays no role in jasmonate biosynthesis Parthenium argentatum
physiological function plays a role in jasmonate biosynthesis Zea mays
physiological function plays a role in jasmonate biosynthesis Solanum tuberosum
physiological function plays a role in jasmonate biosynthesis Solanum lycopersicum