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Literature summary for 4.2.1.91 extracted from

  • Siehl, D.L.; Conn, E.E.
    Kinetic and regulatory properties of arogenate dehydratase in seedlings of Sorghum bicolor (L.) Moench (1988), Arch. Biochem. Biophys., 260, 822-829.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-phenylalanine activity stimulated by, reaction rate 105% Sorghum bicolor
D-tyrosine activity stimulated by, reaction rate 102% Sorghum bicolor
p-hydroxy-phenylacetonitrile activity stimulated by, reaction rate 104% Sorghum bicolor

Inhibitors

Inhibitors Comment Organism Structure
L-phenylalanine competitive inhibition Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
L-arogenate pH 8.0, 22°C, 0.01 mM tyrosine Sorghum bicolor
0.32
-
L-arogenate pH 8.0, 22°C Sorghum bicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arogenate Sorghum bicolor biosynthetic pathway of phenylalanine L-phenylalanine + CO2 + H2O
-
?
L-arogenate Sorghum bicolor ATX2752 biosynthetic pathway of phenylalanine L-phenylalanine + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
ATX2752
-
Sorghum bicolor ATX2752
-
ATX2752
-

Purification (Commentary)

Purification (Comment) Organism
-
Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Sorghum bicolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00425
-
-
Sorghum bicolor

Storage Stability

Storage Stability Organism
-20°C, overnight dialysis against 100 mM Epps, pH 8.0, 20% ethylene glycol, 0.1 mM EDTA, 2 mM DTT, activity is stable for 6 months Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate
-
Sorghum bicolor L-phenylalanine + CO2 + H2O
-
?
L-arogenate biosynthetic pathway of phenylalanine Sorghum bicolor L-phenylalanine + CO2 + H2O
-
?
L-arogenate
-
Sorghum bicolor ATX2752 L-phenylalanine + CO2 + H2O
-
?
L-arogenate biosynthetic pathway of phenylalanine Sorghum bicolor ATX2752 L-phenylalanine + CO2 + H2O
-
?
additional information does not accept prephenate as substrate Sorghum bicolor ?
-
?
additional information does not accept prephenate as substrate Sorghum bicolor ATX2752 ?
-
?

Synonyms

Synonyms Comment Organism
cyclohexadienyl dehydratase
-
Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Sorghum bicolor

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
rate declines at pH 9.0 and above Sorghum bicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.024
-
L-phenylalanine pH 8.0, 22°C Sorghum bicolor