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Literature summary for 4.2.1.90 extracted from

  • Watanabe, S.; Makino, K.
    Novel modified version of nonphosphorylated sugar metabolism-an alternative L-rhamnose pathway of Sphingomonas sp (2009), FEBS J., 276, 1554-1567.
    View publication on PubMed
Search for more literature for 4.2.1.90

Cloned(Commentary)

Cloned (Comment) Organism
gene LRA3, functional expression in Escherichia coli Sphingomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information introduction of genes LRA1-4 or LRA1-3, LRA5 and LAR6 compensats for the L-rhamnose-defective phenotype of an Escherichia coli mutant Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the kcat/Km value with L-rhamnonate is 26.2fold and 59.2fold higher than those with L-lyxonate and L-mannonate, respectively, mainly due to 13.6fold and 30.2fold higher Km values, respectively Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lyxonate Sphingomonas sp. best substrate, 128% activity compared to L-rhamnonate ? + H2O
-
 ?
L-mannonate Sphingomonas sp. 89.2% activity compared to L-rhamnonate ? + H2O
-
 ?
L-rhamnonate Sphingomonas sp. a step in the rhamnose metabolic pathway, overview 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. Q1NEI8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lyxonate best substrate, 128% activity compared to L-rhamnonate Sphingomonas sp. ? + H2O
-
 ?
L-mannonate 89.2% activity compared to L-rhamnonate Sphingomonas sp. ? + H2O
-
 ?
L-rhamnonate
-
 Sphingomonas sp. 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
L-rhamnonate a step in the rhamnose metabolic pathway, overview Sphingomonas sp. 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
LRA3
-
 Sphingomonas sp.