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Literature summary for 4.2.1.84 extracted from

  • Lin, Z.J.; Zheng, R.C.; Wang, Y.J.; Zheng, Y.G.; Shen, Y.C.
    Enzymatic production of 2-amino-2,3-dimethylbutyramide by cyanide-resistant nitrile hydratase (2012), J. Ind. Microbiol. Biotechnol., 39, 133-141.
    View publication on PubMed
Search for more literature for 4.2.1.84

Application

Application Comment Organism
synthesis nitrile hydratase-catalyzed preparation of 2-amino-2,3-dimethylbutyramide, ADBA, a key intermediate for imidazolinone herbicides, method development and optimization, evaluation of the appropriate organism, overview Alcaligenes faecalis
synthesis nitrile hydratase-catalyzed preparation of 2-amino-2,3-dimethylbutyramide, ADBA, a key intermediate for imidazolinone herbicides, method development and optimization, evaluation of the appropriate organism, overview Bacillus subtilis
synthesis nitrile hydratase-catalyzed preparation of 2-amino-2,3-dimethylbutyramide, ADBA, a key intermediate for imidazolinone herbicides, method development and optimization, evaluation of the appropriate organism, overview Serratia marcescens
synthesis nitrile hydratase-catalyzed preparation of 2-amino-2,3-dimethylbutyramide, ADBA, a key intermediate for imidazolinone herbicides, method development and optimization, evaluation of the appropriate organism, overview Rhodococcus sp.
synthesis nitrile hydratase-catalyzed preparation of 2-amino-2,3-dimethylbutyramide, ADBA, a key intermediate for imidazolinone herbicides, method development and optimization, evaluation of the appropriate organism, overview Rhodococcus ruber
synthesis nitrile hydratase-catalyzed preparation of 2-amino-2,3-dimethylbutyramide, ADBA, a key intermediate for imidazolinone herbicides, method development and optimization, evaluation of the appropriate organism, overview Rhodococcus boritolerans

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme from Alcaligenes faecalis strain CCTCC M 208168 is resistant to inhibition by cyanide Alcaligenes faecalis
additional information the enzyme from Bacillus subtilis CCTCC M 206038 is resistant to inhibition by cyanide Bacillus subtilis
additional information the enzyme from Rhodococcus boritolerans strain CCTCC M 208108 is resistant to inhibition by cyanide Rhodococcus boritolerans
additional information the enzyme from Rhodococcus ruber strain CCTCC M 206040 is resistant to inhibition by cyanide Rhodococcus ruber
additional information the enzyme from Rhodococcus sp. {G20} is resistant to inhibition by cyanide; the enzyme from Rhodococcus sp. strain N595 is resistant to inhibition by cyanide; the enzyme from Rhodococcus sp. strain P4 is resistant to inhibition by cyanide; the enzyme from Rhodococcus sp. strain ZA0707 is resistant to inhibition by cyanide Rhodococcus sp.
additional information the enzyme from Serratia marcescens CCTCC M 208231 is resistant to inhibition by cyanide Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-2,3-dimethylbutyronitrile + H2O Alcaligenes faecalis
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Bacillus subtilis
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Serratia marcescens
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Rhodococcus sp.
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Rhodococcus ruber
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Rhodococcus boritolerans
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Alcaligenes faecalis CCTCC M 208168
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Rhodococcus boritolerans CCTCC M 208108
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Bacillus subtilis CCTCC M 206038
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Rhodococcus ruber CCTCC M 206040
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Serratia marcescens CCTCC M 208231
-
 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O Rhodococcus sp. N595
-
 2-amino-2,3-dimethylbutyramide
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis CCTCC M 208168
-
-
-
Bacillus subtilis
-
-
-
Bacillus subtilis CCTCC M 206038
-
-
-
Rhodococcus boritolerans
-
-
-
Rhodococcus boritolerans CCTCC M 208108
-
-
-
Rhodococcus ruber
-
-
-
Rhodococcus ruber CCTCC M 206040
-
-
-
Rhodococcus sp.
-
-
-
Rhodococcus sp. N595
-
-
-
Serratia marcescens
-
-
-
Serratia marcescens CCTCC M 208231
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Alcaligenes faecalis 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Bacillus subtilis 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Serratia marcescens 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Rhodococcus sp. 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Rhodococcus ruber 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Rhodococcus boritolerans 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Alcaligenes faecalis CCTCC M 208168 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Rhodococcus boritolerans CCTCC M 208108 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Bacillus subtilis CCTCC M 206038 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Rhodococcus ruber CCTCC M 206040 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Serratia marcescens CCTCC M 208231 2-amino-2,3-dimethylbutyramide
-
 ?
2-amino-2,3-dimethylbutyronitrile + H2O
-
 Rhodococcus sp. N595 2-amino-2,3-dimethylbutyramide
-
 ?

Synonyms

Synonyms Comment Organism
NHase
-
 Alcaligenes faecalis
NHase
-
 Bacillus subtilis
NHase
-
 Serratia marcescens
NHase
-
 Rhodococcus sp.
NHase
-
 Rhodococcus ruber
NHase
-
 Rhodococcus boritolerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Alcaligenes faecalis
30
-
 assay at Bacillus subtilis
30
-
 assay at Serratia marcescens
30
-
 assay at Rhodococcus sp.
30
-
 assay at Rhodococcus ruber
30
-
 assay at Rhodococcus boritolerans