Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodopseudomonas palustris | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | ? | - |
? | |
additional information | Rhodopseudomonas palustris CGA009 | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
- |
- |
Rhodopseudomonas palustris CGA009 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-difluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2,6-difluorobenzamide | - |
? | |
2,6-difluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris CGA009 | 2,6-difluorobenzamide | - |
? | |
2-bromobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-bromobenzamide | - |
ir | |
2-chlorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-chlorobenzamide | - |
ir | |
2-fluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-fluorobenzamide | - |
ir | |
2-methoxybenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-methoxybenzamide | - |
ir | |
2-methylbenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-methylbenzamide | - |
ir | |
3-(trifluoromethyl)benzonitrile + H2O | 5% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-(trifluoromethyl)benzamide | - |
? | |
3-(trifluoromethyl)benzonitrile + H2O | 5% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris CGA009 | 3-(trifluoromethyl)benzamide | - |
? | |
3-(trifluoromethyl)pyridine-4-carbonitrile + H2O | - |
Rhodopseudomonas palustris | 3-(trifluoromethyl)pyridine-4-carboxamide | - |
ir | |
3-bromobenzonitrile + H2O | 5% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-bromobenzamide | - |
? | |
3-chlorobenzonitrile + H2O | 95% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-chlorobenzamide | - |
? | |
3-fluorobenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-fluorobenzamide | - |
? | |
3-methoxybenzonitrile + H2O | 3% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-methoxybenzamide | - |
? | |
3-methylbenzonitrile + H2O | 17% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-methylbenzamide | - |
? | |
3-phenylpropanenitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-phenylpropanamide | - |
ir | |
4-(trifluoromethyl)benzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-(trifluoromethyl)benzamide | - |
ir | |
4-acetylbenzonitrile + H2O | above 99% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-acetylbenzamide | - |
ir | |
4-bromobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-bromobenzamide | - |
ir | |
4-bromobenzonitrile + H2O | above 99% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-bromobenzamide | - |
? | |
4-chlorobenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-chlorobenzamide | - |
? | |
4-chlorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-chlorbenzamide | - |
ir | |
4-fluorobenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-fluorobenzamide | - |
? | |
4-fluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-fluorbenzamide | - |
ir | |
4-hydroxybenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-hydroxybenzoic acid amide | - |
? | |
4-hydroxybenzonitrile + H2O | - |
Rhodopseudomonas palustris CGA009 | 4-hydroxybenzoic acid amide | - |
? | |
4-hydroxybenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-hydroxybenzamide | - |
? | |
4-methoxybenzonitrile + H2O | above 99% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-methoxybenzamide | - |
ir | |
4-methylbenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-methylbenzamide | - |
ir | |
benzonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | benzamide | - |
ir | |
benzonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris CGA009 | benzamide | - |
ir | |
ethyl 2-cyanobenzoate + H2O | - |
Rhodopseudomonas palustris | ethyl 2-carbamoylbenzoate | - |
ir | |
ethyl 3-cyanobenzoate + H2O | no conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | ethyl 3-carbamoylbenzoate | - |
? | |
ethyl 4-cyanobenzoate + H2O | 95% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | ethyl 4-carbamoylbenzoate | - |
ir | |
furan-2-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | furan-2-carboxamide | - |
ir | |
methyl 4-cyanobenzoate + H2O | 98% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | methyl 4-carbamoylbenzoate | - |
ir | |
additional information | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | Rhodopseudomonas palustris | ? | - |
? | |
additional information | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | Rhodopseudomonas palustris CGA009 | ? | - |
? | |
phenylacetonitrile + H2O | 28% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 2-phenylacetamide | - |
ir | |
pyridine-2-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | pyridine-2-carboxamide | - |
ir | |
pyridine-4-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | pyridine-4-carboxamide | - |
ir | |
thiophen-2-ylacetonitrile + H2O | 89% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 2-(thiophen-2-yl)acetamide | - |
ir | |
thiophene-2-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | thiophene-2-carboxamide | - |
? | |
thiophene-2-carbonitrile + H2O | - |
Rhodopseudomonas palustris | thiophen-2-carboxamide | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Rhodopseudomonas palustris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rhodopseudomonas palustris |