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Literature summary for 4.2.1.84 extracted from
- Immobilization of Geobacillus pallidus RAPc8 nitrile hydratase (NHase) reduces substrate inhibition and enhances thermostability (2010), J. Mol. Catal. B, 63, 109-115.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Aeribacillus pallidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Cyanopyridine | the soluble enzyme shows about 15% residual activity at 500 mM 3-cyanopyridine, NHase immobilized to EupergitC and cross-linked with 1-ethyl-3-(dimethylamino-propyl) carbodiimide shows approximately 50% reduction in 3-cyanopyridine inhibition (about 65% residual activity at 500 mM 3-cyanopyridine) | Aeribacillus pallidus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.3 | - |
2-Cyanopyridine | soluble NHase, at 50°C | Aeribacillus pallidus | |
| 8.7 | - |
4-cyanopyridine | soluble NHase, at 50°C | Aeribacillus pallidus | |
| 10.2 | - |
3-Cyanopyridine | soluble NHase, at 50°C | Aeribacillus pallidus | |
| 17.3 | - |
3-Cyanopyridine | EupergitC-immobilized NHase, at 50°C | Aeribacillus pallidus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 1,4-dioxane | the soluble enzyme shows about 80% relative activity in the presence of 1,4-dioxane | Aeribacillus pallidus |
| Acetone | the soluble enzyme shows about 35% relative activity in the presence of acetone | Aeribacillus pallidus |
| chloroform | the soluble enzyme shows about 70% relative activity in the presence of chloroform | Aeribacillus pallidus |
| dichloromethane | the soluble enzyme shows about 80% relative activity in the presence of dichloromethane | Aeribacillus pallidus |
| Ethanol | the soluble enzyme shows about 60% relative activity in the presence of ethanol | Aeribacillus pallidus |
| formaldehyde dichloromethane | the soluble enzyme shows about 20% relative activity in the presence of formaldehyde | Aeribacillus pallidus |
| Methanol | the soluble enzyme shows about 70% relative activity in the presence of methanol | Aeribacillus pallidus |
| additional information | the soluble enzyme is not influenced by n-hexane, cyclohexane, toluene, benzene, ethyl acetate, and propanol | Aeribacillus pallidus |
| tetrahydrofuran | the soluble enzyme shows about 40% relative activity in the presence of tetrahydrofuran | Aeribacillus pallidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeribacillus pallidus | - |
- |
- |
| Aeribacillus pallidus RAPc8 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Q-Sepharose column chromatography | Aeribacillus pallidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-cyanopyridine + H2O | - |
Aeribacillus pallidus | pyridine-2-carbamide | - |
? | |
| 2-cyanopyridine + H2O | - |
Aeribacillus pallidus RAPc8 | pyridine-2-carbamide | - |
? | |
| 3-cyanopyridine + H2O | - |
Aeribacillus pallidus | pyridine-3-carbamide | - |
? | |
| 3-cyanopyridine + H2O | - |
Aeribacillus pallidus RAPc8 | pyridine-3-carbamide | - |
? | |
| 4-cyanopyridine + H2O | - |
Aeribacillus pallidus | isonicotinamide | - |
? | |
| 4-cyanopyridine + H2O | - |
Aeribacillus pallidus RAPc8 | isonicotinamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NHase | - |
Aeribacillus pallidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aeribacillus pallidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | the activity is not retained for more than 20 min at temperatures above 50°C for the soluble enzyme, at 60°C the half-life of the immobilized NHase (Eupergit C with 1-ethyl-3-(dimethylamino-propyl) carbodiimide) is 330 min as compared with 54.5 min for the soluble enzyme. Eupergit C-immobilized NHase shows 80% residual activity after 90 min at 60°C | Aeribacillus pallidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17.3 | - |
3-Cyanopyridine | EupergitC-immobilized NHase, at 50°C | Aeribacillus pallidus | |
| 59.05 | - |
4-cyanopyridine | soluble NHase, at 50°C | Aeribacillus pallidus | |
| 443.3 | - |
2-Cyanopyridine | soluble NHase, at 50°C | Aeribacillus pallidus | |
| 629.6 | - |
3-Cyanopyridine | soluble NHase, at 50°C | Aeribacillus pallidus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aeribacillus pallidus |
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