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Literature summary for 4.2.1.84 extracted from

  • Yamanaka, Y.; Hashimoto, K.; Ohtaki, A.; Noguchi, K.; Yohda, M.; Odaka, M.
    Kinetic and structural studies on roles of the serine ligand and a strictly conserved tyrosine residue in nitrile hydratase (2010), J. Biol. Inorg. Chem., 15, 655-665.
    View publication on PubMed
Search for more literature for 4.2.1.84

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in Escherichia coli HMS174(DE3)pLysS cells Rhodococcus erythropolis

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzymes S113A and Y72F, hanging drop vapor diffusion method, using 20% (w/v) PEG8000, 0.10 M Tris-HCl, pH 7.5, 0.3 M MgCl2 Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
S113A the mutation partially affects catalytic activity and does not change the pH profiles of the kinetic parameters, the electronic state of the Fe center is altered Rhodococcus erythropolis
Y72F the mutant exhibits no activity Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
n-butyric acid competitive inhibitor Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 Methacrylonitrile wild type enzyme, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis
1.3
-
 Methacrylonitrile mutant enzyme S113A, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ has an unusual Fe3+ center with two modified Cys ligands Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis N771
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methacrylonitrile + H2O
-
 Rhodococcus erythropolis methacrylamide
-
 ?
methacrylonitrile + H2O
-
 Rhodococcus erythropolis N771 methacrylamide
-
 ?

Synonyms

Synonyms Comment Organism
NHase
-
 Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000065
-
 Methacrylonitrile mutant enzyme S113A, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis
0.000016
-
 Methacrylonitrile wild type enzyme, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
 Rhodococcus erythropolis