Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodococcus equi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | a Fe-type NHase | Rhodococcus equi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 24000 + 1 * 23000, SDS-PAGE | Rhodococcus equi |
24000 | - |
1 * 24000 + 1 * 23000, SDS-PAGE | Rhodococcus equi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus equi | - |
- |
- |
Rhodococcus equi TG328-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose 6 column chromatography | Rhodococcus equi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
844 | - |
crude cell extract towards methacrylonitrile, in 50 mM Tris-HCl (pH 7.5) | Rhodococcus equi |
6290 | - |
after 7.5fold purification towards methacrylonitrile, in 50 mM Tris-HCl (pH 7.5) | Rhodococcus equi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-(4-nitrophenyl)-propionitrile + H2O | 39% conversion | Rhodococcus equi | ? | - |
? | |
(R,S)-2-bromopropionitrile + H2O | 47% conversion | Rhodococcus equi | ? | - |
? | |
(R,S)-2-chloropropionitrile + H2O | 48% conversion | Rhodococcus equi | ? | - |
? | |
(R,S)-2-phenylbutyronitrile + H2O | 51% conversion | Rhodococcus equi | ? | - |
? | |
(R,S)-2-phenylpropionitrile + H2O | 43% conversion | Rhodococcus equi | ? | - |
? | |
(R,S)-3-oxo-2-phenylbutyronitrile + H2O | 43% conversion | Rhodococcus equi | ? | - |
? | |
2-phenylacetonitrile + H2O | - |
Rhodococcus equi | 2-phenylpropionamide | - |
? | |
3-cyanopyridine + H2O | - |
Rhodococcus equi | pyridine-3-carbamide | - |
? | |
benzonitrile + H2O | - |
Rhodococcus equi | benzoic acid amide | - |
? | |
benzonitrile + H2O | - |
Rhodococcus equi TG328-2 | benzoic acid amide | - |
? | |
isobutyronitrile + H2O | - |
Rhodococcus equi | isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | - |
Rhodococcus equi TG328-2 | isobutyric acid amide | - |
? | |
methacrylonitrile + H2O | - |
Rhodococcus equi | methacrylamide | - |
? | |
additional information | besides aromatic and heterocyclic nitriles, aliphatic ones are hydrated preferentially | Rhodococcus equi | ? | - |
? | |
additional information | no activity with (R,S)-2-(4-isobutylphenyl)propionitrile and (R,S)-3-(1-cyanoethyl)benzoic acid | Rhodococcus equi | ? | - |
? | |
additional information | besides aromatic and heterocyclic nitriles, aliphatic ones are hydrated preferentially | Rhodococcus equi TG328-2 | ? | - |
? | |
additional information | no activity with (R,S)-2-(4-isobutylphenyl)propionitrile and (R,S)-3-(1-cyanoethyl)benzoic acid | Rhodococcus equi TG328-2 | ? | - |
? | |
valeronitrile + H2O | almost 9000fold higher activity towards valeronitrile compared to (R,S)-2-phenylpropionitrile | Rhodococcus equi | n-pentanoic acid amide | - |
? | |
valeronitrile + H2O | almost 9000fold higher activity towards valeronitrile compared to (R,S)-2-phenylpropionitrile | Rhodococcus equi TG328-2 | n-pentanoic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 24000 + 1 * 23000, SDS-PAGE | Rhodococcus equi |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Rhodococcus equi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodococcus equi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 70 | highest stability at 4°C in thermal inactivation studies between 4°C and 50°C, the enzyme is inactivated immediately at 70°C | Rhodococcus equi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Rhodococcus equi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Rhodococcus equi |