KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics | Rhodococcus erythropolis | |
additional information | - |
additional information | thermodynamics | Pseudonocardia thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co3+ | the enzyme belongs to the CoIII-NHase group of enzymes, octahedrally coordinated metal ion with two deprotonated backbone amides as ligands as well as three cysteine residues, two of which are posttranslationally oxidized to cysteine-sulfenic and cysteine-sulfinic acids | Pseudonocardia thermophila | |
Fe3+ | the enzyme belongs to the FeIII-NHase group of enzymes, octahedrally coordinated metal ion with two deprotonated backbone amides as ligands as well as three cysteine residues, two of which are posttranslationally oxidized to cysteine-sulfenic and cysteine-sulfinic acids | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aliphatic amide | Rhodococcus erythropolis | - |
a nitrile + H2O | - |
? | |
an aliphatic amide | Pseudonocardia thermophila | - |
a nitrile + H2O | - |
? | |
an aliphatic amide | Rhodococcus erythropolis N-771 | - |
a nitrile + H2O | - |
? | |
an aliphatic amide | Pseudonocardia thermophila JCM 3095 | - |
a nitrile + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudonocardia thermophila | - |
- |
- |
Pseudonocardia thermophila JCM 3095 | - |
- |
- |
Rhodococcus erythropolis | - |
- |
- |
Rhodococcus erythropolis N-771 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aliphatic amide = a nitrile + H2O | first- and second-shell reaction mechanism, a tyrosine residue acts as catalytic base, modelling, detailed overview | Rhodococcus erythropolis | |
an aliphatic amide = a nitrile + H2O | reaction mechanism, first-shell mechanism of CoIII-NHase involving Tyr68 as catalytic base, deprotonated Tyr68 is proposed to abstract a proton from the nucleophilic water molecule, thus activating it for attack on the metal-bound substrate, modelling, detailed overview | Pseudonocardia thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aliphatic amide | - |
Rhodococcus erythropolis | a nitrile + H2O | - |
? | |
an aliphatic amide | - |
Pseudonocardia thermophila | a nitrile + H2O | - |
? | |
an aliphatic amide | ligand exchange reactions, overview | Rhodococcus erythropolis | a nitrile + H2O | - |
? | |
an aliphatic amide | ligand exchange reactions, overview | Pseudonocardia thermophila | a nitrile + H2O | - |
? | |
an aliphatic amide | - |
Rhodococcus erythropolis N-771 | a nitrile + H2O | - |
? | |
an aliphatic amide | ligand exchange reactions, overview | Rhodococcus erythropolis N-771 | a nitrile + H2O | - |
? | |
an aliphatic amide | - |
Pseudonocardia thermophila JCM 3095 | a nitrile + H2O | - |
? | |
an aliphatic amide | ligand exchange reactions, overview | Pseudonocardia thermophila JCM 3095 | a nitrile + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CoIII-NHase | - |
Rhodococcus erythropolis |
CoIII-NHase | - |
Pseudonocardia thermophila |