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Literature summary for 4.2.1.84 extracted from

  • Yu, H.; Shi, Y.; Luo, H.; Tian, Z.; Zhu, Y.; Shen, Z.
    An over expression and high efficient mutation system of a cobalt-containing nitrile hydratase (2006), J. Mol. Catal. B, 43, 80-85.
No PubMed abstract available
Search for more literature for 4.2.1.84

Cloned(Commentary)

Cloned (Comment) Organism
Nocardia sp.(strain YS-2002) NHase gene with modified start codon in alpha subunit is overexpressed in Escherichia coli, strain BL21(DE3) Nocardia sp.

Protein Variants

Protein Variants Comment Organism
S122A alpha subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.
S122C alpha subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.
S122D alpha subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.
W47E beta subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.

Inhibitors

Inhibitors Comment Organism Structure
Acrylamide mutant enzyme S122C shows higher tolerance under increasing acrylamide levels than recombinant NHase with modified start codon only Nocardia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
82
-
 acrylonitrile recombinant NHase with modified start codon only Nocardia sp.
86
-
 acrylonitrile wild type NHase Nocardia sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp. Q8GJG6 alpha-subunit
-
Nocardia sp. Q8GJG7 beta-subunit
-
Nocardia sp. YS-2002 Q8GJG6 alpha-subunit
-
Nocardia sp. YS-2002 Q8GJG7 beta-subunit
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no activity in S122A and S122D mutant proteins Nocardia sp.
94.3
-
 W47E strain 1 Nocardia sp.
96.8
-
 W47E strain 2 Nocardia sp.
128.7
-
 S122C strain 2 Nocardia sp.
133.2
-
 S122C strain 1 Nocardia sp.
189.2
-
 control strain 1 Nocardia sp.
195.1
-
 control strain 2 Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
 Nocardia sp. acrylamide
-
 ?
acrylonitrile + H2O
-
 Nocardia sp. YS-2002 acrylamide
-
 ?

Synonyms

Synonyms Comment Organism
NHase
-
 Nocardia sp.
nitrile hydratase
-
 Nocardia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 reaction activation energy of the recombinant NHase with modified start codon is 24.4 +/- 0.5 kJ/mol, this is 37.2% of wild type NHase Nocardia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 37 mutant protein W47E shows higher thermo-stability than recombinant NHase with modified start codon only Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8.5 wild type NHase Nocardia sp.
5.5 7.5 recombinant NHase with modified start codon only Nocardia sp.