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Literature summary for 4.2.1.84 extracted from

  • Brady, D.; Beeton, A.; Zeevaart, J.; Kgaje, C.; van Rantwijk, F.; Sheldon, R.A.
    Characterisation of nitrilase and nitrile hydratase biocatalytic systems (2004), Appl. Microbiol. Biotechnol., 64, 76-85.
    View publication on PubMed
Search for more literature for 4.2.1.84

Application

Application Comment Organism
synthesis production of alpha-hydroxy nitriles by use of enzyme Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Rhodococcus sp.
-
-
-
Rhodococcus sp.
-
 strain ATCC BAA-869
-
Rhodococcus sp.
-
 strain ATCC BAA-870
-
Rhodococcus sp.
-
 strain DSM 44519
-
Rhodococcus sp. Novo SP361
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorobenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens (R)-2-chloromandelonitrile 90% conversion to alpha-hydroxy nitrile ?
2-fluorobenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 100% conversion to alpha-hydroxy nitrile ?
2-nitrobenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 20% conversion to alpha-hydroxy nitrile ?
2-phenylbutyronitrile + H2O used as well as phenylacetonitrile Pseudomonas fluorescens 2-phenylbutyramide
-
 ?
2-phenylglycinonitrile + H2O used as well as phenylacetonitrile Pseudomonas fluorescens aminoacetamide
-
 ?
2-phenylpropionitrile + H2O
-
 Rhodococcus sp. 2-phenylpropionamide
-
 ?
2-phenylpropionitrile + H2O used as well as phenylacetonitrile Pseudomonas fluorescens 2-phenylpropionamide
-
 ?
2-phenylpropionitrile + H2O
-
 Rhodococcus sp. Novo SP361 2-phenylpropionamide
-
 ?
3,4-dimethoxybenzonitrile + H2O second step with amidase (EC 3.5.1.4) Rhodococcus sp. 3,4-dimethoxybenzaldehyde + NH3
-
 ?
3,4-dimethoxybenzonitrile + H2O second step with amidase (EC 3.5.1.4) Rhodococcus sp. Novo SP361 3,4-dimethoxybenzaldehyde + NH3
-
 ?
3-chlorobenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 100% conversion to alpha-hydroxy nitrile ?
3-hydroxy-3-phenylpropionitrile + H2O
-
 Rhodococcus sp. 3-hydroxy-3-phenylpropionamide
-
 ?
3-hydroxy-3-phenylpropionitrile + H2O
-
 Rhodococcus sp. Novo SP361 3-hydroxy-3-phenylpropionamide
-
 ?
3-phenylpropionitrile + H2O used as well as phenylacetonitrile Pseudomonas fluorescens 3-phenylpropionamide
-
 ?
4-chlorobenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 100% conversion to alpha-hydroxy nitrile ?
4-cyanobenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 100% conversion to alpha-hydroxy nitrile ?
4-hydroxybenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 55% conversion to alpha-hydroxy nitrile ?
4-methylbenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 51% conversion to alpha-hydroxy nitrile ?
4-nitrobenzaldehyde + HCN + H2O
-
 Pseudomonas fluorescens ? + H2O 100% conversion to alpha-hydroxy nitrile ?
benzaldehyde + HCN
-
 Pseudomonas fluorescens ? + H2O 95% conversion to alpha-hydroxy nitrile ?
mandelonitrile + H2O
-
 Rhodococcus sp. ?
-
 ?
mandelonitrile + H2O used as well as phenylacetonitrile Pseudomonas fluorescens ?
-
 ?
N-phenylglycinenitrile + H2O
-
 Rhodococcus sp. ?
-
 ?
phenylacetonitrile + H2O
-
 Pseudomonas fluorescens phenylacetamide
-
 ?
phenylacetonitrile + H2O
-
 Rhodococcus sp. phenylacetamide
-
 ?
phthalonitrile + H2O
-
 Rhodococcus sp. ?
-
 ?