Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
Methacrylonitrile | pH 7.2, 25ºC | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co3+ | CD spectrum suggests low-spin Co3+ in tetragonally-distorted octahedral ligand field | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22982 | - |
1 * 22982, alpha + 1* 24108, beta, amino acid sequence | Pseudomonas putida |
22982 | - |
2 * 22982, alpha + 2 * 24108, beta, amino acid sequence, gel filtration | Pseudomonas putida |
24108 | - |
2 * 22982, alpha + 2 * 24108, beta, amino acid sequence, gel filtration | Pseudomonas putida |
54000 | - |
chromatography on Sephacryl S-400 and Sepharose CL-6B, alpha-beta form | Pseudomonas putida |
94000 | - |
gel chromatography on Superdex 200 , (alpha-beta)2 form | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida NRRL-18668 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatography on DEAE-Cellulose, hydroxyapatite, phenyl-Sepharose and Sephacryl S-400 | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1590 | - |
purified enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O | enantioselective hydration | Pseudomonas putida | 2(S)-(4-chloro-phenyl)-3-methyl-butyramide | - |
? | |
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O | enantioselective hydration | Pseudomonas putida NRRL-18668 | 2(S)-(4-chloro-phenyl)-3-methyl-butyramide | - |
? | |
methacrylonitrile + H2O | - |
Pseudomonas putida | methylacrylic acid amide | - |
? | |
methacrylonitrile + H2O | - |
Pseudomonas putida NRRL-18668 | methylacrylic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Pseudomonas putida |
dimer | 1 * 22982, alpha + 1* 24108, beta, amino acid sequence | Pseudomonas putida |
tetramer | 2 * 22982, alpha + 2 * 24108, beta, amino acid sequence, gel filtration | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
1 hour, 10 mM methacrylonitrile stable up to this temperature | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | - |
Pseudomonas putida |