KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values for 12 different substrates, affinity increases as the side chain of the substrate becomes longer | Brevibacterium sp. | |
25 | - |
acetonitrile | pH 7, 30°C | Brevibacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sp. | - |
- |
- |
Brevibacterium sp. R312 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cell-free extract preparation | Brevibacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminopropionitrile + H2O | 90% of the activity with propionitrile | Brevibacterium sp. | 2-aminopropionic acid amide | - |
? | |
2-hydroxypropionitrile + H2O | i.e. DL-lactonitrile | Brevibacterium sp. | 2-hydroxypropionic acid amide | - |
? | |
2-hydroxypropionitrile + H2O | 116% of the activity with propionitrile | Brevibacterium sp. | 2-hydroxypropionic acid amide | - |
? | |
3-aminopropionitrile + H2O | 2.7% of the activity with propionitrile | Brevibacterium sp. | 3-aminopropionic acid amide | - |
? | |
3-hydroxypropionitrile + H2O | 35% of the activity with propionitrile | Brevibacterium sp. | 3-hydroxypropanamide | - |
? | |
acetonitrile + H2O | 10% of the activity with propionitrile | Brevibacterium sp. | acetamide | - |
? | |
acetonitrile + H2O | 10% of the activity with propionitrile | Brevibacterium sp. R312 | acetamide | - |
? | |
acrylonitrile + H2O | - |
Brevibacterium sp. | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
benzonitrile + H2O | 1.3% of the activity with propionitrile | Brevibacterium sp. | benzoic acid amide | - |
? | |
cyanide + H2O | 2% of the activity with propionitrile | Brevibacterium sp. | formamide | - |
? | |
isobutyronitrile + H2O | 113% of the activity with propionitrile | Brevibacterium sp. | isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | 113% of the activity with propionitrile | Brevibacterium sp. R312 | isobutyric acid amide | - |
? | |
n-butyronitrile + H2O | 33% of the activity with propionitrile | Brevibacterium sp. | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | 33% of the activity with propionitrile | Brevibacterium sp. R312 | n-butyric acid amide | - |
? | |
pivalonitrile + H2O | 5.3% of the activity with propionitrile | Brevibacterium sp. | 2,2-dimethylpropionic acid amide | - |
? | |
propionitrile + H2O | - |
Brevibacterium sp. | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Brevibacterium sp. R312 | propionic acid amide | - |
? | |
valeronitrile + H2O | 2.7% of the activity with propionitrile | Brevibacterium sp. | n-pentanoic acid amide | - |
? | |
valeronitrile + H2O | 2.7% of the activity with propionitrile | Brevibacterium sp. R312 | n-pentanoic acid amide | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | optimum temperature for acetonitrile hydrolysis | Brevibacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Brevibacterium sp. |