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Literature summary for 4.2.1.84 extracted from

  • Hjort, C.M.; Godtfredsen, S.E.; Emborg, C.
    Isolation and characterization of a nitrile hydratase from Rhodococcus sp. (1990), J. Chem. Technol. Biotechnol., 48, 217-226.
No PubMed abstract available
Search for more literature for 4.2.1.84

Application

Application Comment Organism
synthesis useful for acrylamide production Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Rhodococcus sp.
Cu2+ 1-5 mM CuSO4 Rhodococcus sp.
EDTA weak Rhodococcus sp.
Hg2+ complete inactivation at 0.05 mM Hg2+; HgCl2, 0.01-1 mM Rhodococcus sp.
iodoacetic acid
-
 Rhodococcus sp.
Propionamide weak inactivation Rhodococcus sp.
thiocyanate weak Rhodococcus sp.
Zn2+ weak; ZnCl2, 1 mM Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 Benzonitrile pH 7, 37°C Rhodococcus sp.
6
-
 Propionitrile pH 7, 37°C Rhodococcus sp.
14
-
 acrylonitrile pH 7, 37°C Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 23000 + 1 * 26000, SDS-PAGE Rhodococcus sp.
26000
-
 1 * 23000 + 1 * 26000, SDS-PAGE Rhodococcus sp.
52000
-
 gel filtration Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
867
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetonitrile + H2O
-
 Rhodococcus sp. acetamide
-
 ?
acrylonitrile + H2O
-
 Rhodococcus sp. 2-propenoic acid amide i.e. acrylic acid amide ?
benzonitrile + H2O
-
 Rhodococcus sp. benzoic acid amide
-
 ?
cyanide + H2O
-
 Rhodococcus sp. formamide
-
 ?
n-butyronitrile + H2O
-
 Rhodococcus sp. n-butyric acid amide
-
 ?
propionitrile + H2O
-
 Rhodococcus sp. propionic acid amide
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 23000 + 1 * 26000, SDS-PAGE Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 stable below Rhodococcus sp.
25
-
 5 min, unstable above Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8.3 about 50% of activity maximum at pH 6, about 35% of activity maximum at pH 8.3 Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 highly unstable below pH 7.0 Rhodococcus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 Propionamide pH 7, 37°C, mixed-type inhibitor Rhodococcus sp.
8.4
-
 thiocyanate pH 7, 37°C, competitive inhibitor Rhodococcus sp.