Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0317 | - |
2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
0.0386 | - |
2-hydroxypent-2,4-dienoate | activated by Mn2+ | Paraburkholderia xenovorans | |
0.0408 | - |
2-hydroxyhexa-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
0.0459 | - |
5-chloro-2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
Co2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
Mg2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
Mn2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
Zn2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
8 * 27000, SDS-PAGE | Paraburkholderia xenovorans |
221000 | - |
gel filtration | Paraburkholderia xenovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxypent-2,4-dienoate + H2O | Paraburkholderia xenovorans | reaction in the catabolic pathway of numerous aromatic compounds, including polychlorinated biphenyls | 2-keto-4-hydroxypentanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
LB400 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyhexa-2,4-dienoate + H2O | - |
Paraburkholderia xenovorans | 2-keto-4-hydroxyhexanoate | - |
? | |
2-hydroxypent-2,4-dienoate + H2O | - |
Paraburkholderia xenovorans | 2-keto-4-hydroxypentanoate | - |
? | |
2-hydroxypent-2,4-dienoate + H2O | reaction in the catabolic pathway of numerous aromatic compounds, including polychlorinated biphenyls | Paraburkholderia xenovorans | 2-keto-4-hydroxypentanoate | - |
? | |
5-chloro-2-hydroxypent-2,4-dienoate | - |
Paraburkholderia xenovorans | 5-chloro-2-keto-4-hydroxypentanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 27000, SDS-PAGE | Paraburkholderia xenovorans |
Synonyms | Comment | Organism |
---|---|---|
BphH | - |
Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
5-chloro-2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
60.5 | - |
2-hydroxyhexa-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
215 | - |
2-hydroxypent-2,4-dienoate | activated by Mn2+ | Paraburkholderia xenovorans | |
361 | - |
2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Paraburkholderia xenovorans |