Cloned (Comment) | Organism |
---|---|
gene prpD in the operon prpBCDE, DNA sequence determination nand analysis, overexpression as N-terminally His-tagged protein in Escherichia coli BL21(DE3) | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ions and reducing agents | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Hydroxybutane-1,2,3-tricarboxylate | Salmonella enterica | - |
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? | |
additional information | Salmonella enterica | the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | P74840 | prpBCDE operon; serovar Typhimurium LT2, gene prpD of the prpBCDE operon | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli BL21(DE3), to homogeneity | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
purified recombinant His-tagged enzyme, substrate citrate | Salmonella enterica |
2.6 | - |
purified recombinant His-tagged enzyme, substrate cis-aconitate | Salmonella enterica |
2.8 | - |
purified recombinant His-tagged enzyme, substrate 2-methylcitrate | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Hydroxybutane-1,2,3-tricarboxylate | - |
Salmonella enterica | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? | |
2-Hydroxybutane-1,2,3-tricarboxylate | i.e. 2-methylcitrate | Salmonella enterica | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | i.e. 2-methyl-cis-aconitate | ? | |
cis-aconitate | - |
Salmonella enterica | ? | - |
? | |
citrate | low activity with | Salmonella enterica | ? | - |
? | |
additional information | enzyme does not catalyze the hydration of 2-methyl-cis-aconitate to 2-methylisocitrate, isocitrate is no substrate | Salmonella enterica | ? | - |
? | |
additional information | the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview | Salmonella enterica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PrpD | - |
Salmonella enterica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salmonella enterica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains no iron-sulfur center | Salmonella enterica |