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Literature summary for 4.2.1.79 extracted from

  • Horswill, A.R.; Escalante-Semerena, J.C.
    In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate (2001), Biochemistry, 40, 4703-4713.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene prpD in the operon prpBCDE, DNA sequence determination nand analysis, overexpression as N-terminally His-tagged protein in Escherichia coli BL21(DE3) Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions and reducing agents Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate Salmonella enterica
-
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
?
additional information Salmonella enterica the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica P74840 prpBCDE operon; serovar Typhimurium LT2, gene prpD of the prpBCDE operon
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli BL21(DE3), to homogeneity Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
purified recombinant His-tagged enzyme, substrate citrate Salmonella enterica
2.6
-
purified recombinant His-tagged enzyme, substrate cis-aconitate Salmonella enterica
2.8
-
purified recombinant His-tagged enzyme, substrate 2-methylcitrate Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Hydroxybutane-1,2,3-tricarboxylate
-
Salmonella enterica (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
?
2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate Salmonella enterica (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate ?
cis-aconitate
-
Salmonella enterica ?
-
?
citrate low activity with Salmonella enterica ?
-
?
additional information enzyme does not catalyze the hydration of 2-methyl-cis-aconitate to 2-methylisocitrate, isocitrate is no substrate Salmonella enterica ?
-
?
additional information the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview Salmonella enterica ?
-
?

Synonyms

Synonyms Comment Organism
PrpD
-
Salmonella enterica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme contains no iron-sulfur center Salmonella enterica