Literature summary for 4.2.1.78 extracted from

Lee, E.J.; Facchini, P.
Norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family (2010), Plant Cell, 22, 3489-3503.

Search for more literature for 4.2.1.78

Cloned(Commentary)

Cloned (Comment)	Organism
NCS2-green fluorescent protein fusion introduced by microprojectile bombardment into opium poppy cells initially localizes to the endoplasmic reticulum but subsequently sorts to the vacuole	Papaver somniferum

Protein Variants

Protein Variants	Comment	Organism
additional information	virus-induced gene silencing of NCS in opium poppy profoundly reduces alkaloid levels compared to controls, a mixed culture of Agrobacterium tumefaciens harboring either pTRV2-PsNCS or pTRV1 is infiltrated into opium poppy seedlings tow weeks after germination. Levels of the five major alkaloids in opium poppy latex, i.e. morphine, codeine, thebaine, noscapine, and papaveine, are reduced by 75-82% in plants infiltrated with Agrobacterium tumefaciens harboring pTRV2-PsNCS	Papaver somniferum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	immunoprecipitation of NCS from total protein extracts of Thalictrum flavum cells results in a nearly complete attenuation of NCS activity	Thalictrum flavum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	immunolocalization study of NCS, overview	Papaver somniferum	-	-
soluble	-	Thalictrum flavum	-	-
vacuole	NCS2 has an N-terminal signal peptide and sorts to the vacuole	Papaver somniferum	5773	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol	Papaver somniferum	4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine	(S)-norcoclaurine + H2O	-	?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol	Coptis japonica	4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine	(S)-norcoclaurine + H2O	-	?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol	Thalictrum flavum	4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine	(S)-norcoclaurine + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Coptis japonica	-	-	-
Papaver somniferum	-	isozymes NCS1 and NCS2	-
Thalictrum flavum	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
carpel	low level	Papaver somniferum	-
cell culture	-	Thalictrum flavum	-
flower bud	very low expression level	Thalictrum flavum	-
leaf	low level	Papaver somniferum	-
additional information	not in leaves	Thalictrum flavum	-
phloem	NCS2 shows cell type-specific occurrence in phloem sieve elements, colocalization with all other known benzylisoquinoline alkaloid biosynthetic enzymes	Papaver somniferum	-
rhizome	-	Thalictrum flavum	-
root	-	Thalictrum flavum	-
root	abundant	Papaver somniferum	-
stem	abundant	Papaver somniferum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol	4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine	Papaver somniferum	(S)-norcoclaurine + H2O	-	?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol	4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine	Coptis japonica	(S)-norcoclaurine + H2O	-	?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol	4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine	Thalictrum flavum	(S)-norcoclaurine + H2O	-	?
additional information	isozyme NCS1 of Coptis japonica does not catalyze the formation of (S)-norcoclaurine from dopamine and 4-hydroxyphenylacetaldehyde	Coptis japonica	?	-	?

Synonyms

Synonyms	Comment	Organism
More	norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family	Papaver somniferum
More	norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family	Coptis japonica
More	norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family	Thalictrum flavum
NCS	-	Papaver somniferum
NCS	-	Coptis japonica
NCS	-	Thalictrum flavum
NCS1	-	Papaver somniferum
NCS1	-	Coptis japonica
NCS2	-	Papaver somniferum
norcoclaurine synthase	-	Papaver somniferum
norcoclaurine synthase	-	Coptis japonica
norcoclaurine synthase	-	Thalictrum flavum
PR10A	-	Coptis japonica

Expression

Organism	Comment	Expression
Papaver somniferum	elicitor-induced cell cultures show increasing expression of NCS after 2 to 100 hours	up

General Information

General Information	Comment	Organism
metabolism	norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview	Papaver somniferum
metabolism	norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview	Coptis japonica
metabolism	norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview	Thalictrum flavum
additional information	amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview	Papaver somniferum
additional information	amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview	Coptis japonica
additional information	amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview	Thalictrum flavum

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Release 2023.1 (January 2023)