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Literature summary for 4.2.1.78 extracted from

  • Pasquo, A.; Bonamore, A.; Franceschini, S.; Macone, A.; Boffi, A.; Ilari, A.
    Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum (2008), Acta Crystallogr. Sect. F, 64, 281-283.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the NCS protein truncated at the first 19 amino acids with an N-terminal MTGS sequence and a His tag at the C-terminus and its SeMet-substituted variant are subcloned into the NdeI and XhoI restriction sites of the vector pET22b. Transformation of chemically competent Escherichia coli strain BL21DE is performed and transformed cells are plated onto LB-ampicillin plates Thalictrum flavum

Crystallization (Commentary)

Crystallization (Comment) Organism
at 294 K by the hanging-drop vapour-diffusion method using ammonium sulfate and sodium chloride as precipitant agents and diffract to better than 3.0 A resolution using a synchrotron-radiation source. The crystals belong to the trigonal space group P3121, with unit-cell parameters a = b = 86.31, c = 118.36 A. A selenomethionine derivate is crystallized in the same space group, 2.7 A resolution Thalictrum flavum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde Thalictrum flavum
-
(S)-Norcoclaurine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Thalictrum flavum Q67A25
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap Fast Flow column Thalictrum flavum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde
-
Thalictrum flavum (S)-Norcoclaurine + H2O
-
?

Synonyms

Synonyms Comment Organism
NCS
-
Thalictrum flavum
norcoclaurine synthase
-
Thalictrum flavum