Cloned (Comment) | Organism |
---|---|
the NCS protein truncated at the first 19 amino acids with an N-terminal MTGS sequence and a His tag at the C-terminus and its SeMet-substituted variant are subcloned into the NdeI and XhoI restriction sites of the vector pET22b. Transformation of chemically competent Escherichia coli strain BL21DE is performed and transformed cells are plated onto LB-ampicillin plates | Thalictrum flavum |
Crystallization (Comment) | Organism |
---|---|
at 294 K by the hanging-drop vapour-diffusion method using ammonium sulfate and sodium chloride as precipitant agents and diffract to better than 3.0 A resolution using a synchrotron-radiation source. The crystals belong to the trigonal space group P3121, with unit-cell parameters a = b = 86.31, c = 118.36 A. A selenomethionine derivate is crystallized in the same space group, 2.7 A resolution | Thalictrum flavum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | Thalictrum flavum | - |
(S)-Norcoclaurine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thalictrum flavum | Q67A25 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap Fast Flow column | Thalictrum flavum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | - |
Thalictrum flavum | (S)-Norcoclaurine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NCS | - |
Thalictrum flavum |
norcoclaurine synthase | - |
Thalictrum flavum |