Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisae | Arabidopsis thaliana |
expression in Saccharomyces cerevisae. Recombinant His6-tagged RHM2-N protein (amino acids 1-370). The RHM2/MUM4 protein is more stable than RHM1 or RHM3 in yeast cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D96N | mutation completely abolishes UDP-Glc 4,6-dehydratase activity | Arabidopsis thaliana |
G18A | mutation completely abolishes UDP-Glc 4,6-dehydratase activity | Arabidopsis thaliana |
G193R | mutation completely abolishes UDP-Glc 4,6-dehydratase activity | Arabidopsis thaliana |
K165A | mutation completely abolishes UDP-Glc 4,6-dehydratase activity | Arabidopsis thaliana |
K35A | activity of the mutant is lower than activity of wild-type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | 1 mM, 41% inhibition of purified His6-tagged RHM2-N protein is inhibited | Arabidopsis thaliana | |
UDP-Rha | 1 mM, 82% inhibition of purified His6-tagged RHM2-N protein | Arabidopsis thaliana | |
UDP-Xyl | 1 mM, 79% inhibition of purified His6-tagged RHM2-N protein is inhibited | Arabidopsis thaliana | |
UMP | 1 mM, 23% inhibition of purified His6-tagged RHM2-N protein is inhibited | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.116 | - |
UDP-D-glucose | recombinant RHM2-N protein (amino acids 1370) | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
RHM2/MUM4 is a trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities. The N-terminal region of RHM2 (RHM2-N: amino acids 1-370) has the first activity and the C-terminal region of RHM2 (RHM2-C: amino acids 371-667) has the two following activities | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose | - |
Arabidopsis thaliana | UDP-4-dehydro-6-deoxy-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RHM1 | trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities | Arabidopsis thaliana |
RHM2/MUM4 | trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities. The N-terminal region of RHM2 (RHM2-N, amino acids 1-370) has the first activity and the C-terminal region of RHM2 (RHM2-C, amino acids 371-667) has the two following activities | Arabidopsis thaliana |
RHM3 | trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities | Arabidopsis thaliana |
UDP-Glc 4,6-dehydratase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | 25°C: about 50% of maximal activity, 45°C: about 40% of maximal activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2860 | - |
UDP-D-glucose | recombinant RHM2-N protein (amino acids 1370) | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
the highest level of enzyme activity is observed in 250 mM MOPS-NaOH buffer, pH 7.5. In 250 mM Tris-HCl buffer, pH 7.5, the relative activity is lower than that observed for MOPS-NaOH buffer at the same pH | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 40% of maximal activity, pH 8.0: about 80% of maximal activity | Arabidopsis thaliana |