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Literature summary for 4.2.1.75 extracted from
- Human uroporphyrinogen III synthase: NMR-based mapping of the active site (2007), Proteins, 71, 855-873.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
| expression of N-terminally His-tagged enzyme in Escherichia coli strain strain BL21(DE3) | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| 10% glycerol stabilizes the purified recombinant enzyme in 20 mM sodium phosphate, pH 7.45, 100 mM NaCl, 2 mM DTT, and 0.1 mM EDTA at -80°C | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28712 | - |
x * 28712, calculated from sequence | Homo sapiens |
| 28810 | - |
mass spectrometry | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxymethylbilane | Homo sapiens | the enzyme catalyzes the cyclization and D-ring isomerization of hydroxymethylbilane to uroporphyrinogen III, the cyclic tetrapyrrole and physiologic precursor of heme, chlorophyl, and corrin | uroporphyrinogen III + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P10746 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| recombinant N-terminally His-tagged enzyme from Escherichia coli strain strain BL21(DE3) by ultrafiltration, nickel affinity and anion exchange chromatography, followed by gel filtration to homogeneity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxymethylbilane | - |
Homo sapiens | uroporphyrinogen III + H2O | - |
? | |
| hydroxymethylbilane | the enzyme catalyzes the cyclization and D-ring isomerization of hydroxymethylbilane to uroporphyrinogen III, the cyclic tetrapyrrole and physiologic precursor of heme, chlorophyl, and corrin | Homo sapiens | uroporphyrinogen III + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28712, calculated from sequence | Homo sapiens |
| More | mapping of the URO-synthase active site by NMR perturbation studies, modelling by by in silico docking, active site structure, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| URO-synthase | - |
Homo sapiens |
| Uroporphyrinogen III synthase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 7.4, t1/2: more than 14 days | Homo sapiens |
| 45 | - |
pH 7.4, t1/2: 1.9 days | Homo sapiens |
| 60 | - |
pH 8.2, t1/2: less than 1 day | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Homo sapiens |
| 8.2 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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