Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C73A | 99% of wild-type activity | Homo sapiens |
C73R | 9% of wild-type activity | Homo sapiens |
C73S | 78% of wild-type activity | Homo sapiens |
General Stability | Organism |
---|---|
activity does not change after several cycles of freeze-and thawing in potassium phosphate buffer, pH 7.4, containing DTT and glycerol | Homo sapiens |
recombinant enzyme in cell lysate is unstable, glycerol stabilizes | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.01 | - |
Hydroxymethylbilane | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28807 | - |
x * 28807, MALDI-TOF mass spectrometry | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P10746 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.7 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxymethylbilane | - |
Homo sapiens | uroporphyrinogen III + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28807, MALDI-TOF mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
uro'gen III synthase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme shows remarkable thermostability, particularly when kept in phosphate buffer containing DTT and EDTA, indicating that the activity may depend on its oxidation state | Homo sapiens |
37 | - |
about 20% loss of activity after 48 h, about 50% loss of activity after 72 h, buffer containing 1 mM DTT | Homo sapiens |
45 | - |
about 10% loss of activity after 24 h, buffer containing 1 mM DTT | Homo sapiens |
60 | - |
about 75% loss of activity after 3 min, buffer containing 1 mM DTT | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
Hydroxymethylbilane | - |
Homo sapiens |