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Literature summary for 4.2.1.66 extracted from
- The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat (2009), Appl. Microbiol. Biotechnol., 82, 271-278.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli, pET26b construct p3460, BL21(lambda-DE3) pLysS cells | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, gel filtration and SDS-PAGE | Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
three-dimensional protein reconstruction, negative stain electron microscopy, three-dimensional reconstruction, comparative modeling and docking, recombinant cyanide hydratase fibers analyzed by iterative helical real space reconstruction, determination of left-handed D1 S5.4 symmetry with helical rise of 1.36 nm, different arrangement from other microbial nitrilases that display a reduced helical twist, assembly stabilized by two dyadic interactions between dimers across the one-start helical groove, stereoview of the docked model | Neurospora crassa |
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Release 2023.1 (January 2023)
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