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Literature summary for 4.2.1.66 extracted from

  • Cluness, M.J.; Turner, P.; Clements, E.; Brown, D.T.; O'Reilly, C.
    Purification and properties of cyanide hydratase from Fusarium lateritium and analysis of the corresponding chy1 gene (1993), J. Gen. Microbiol., 139, 1807-1815.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fusarium lateritium

Inhibitors

Inhibitors Comment Organism Structure
CN- rapid inactivation above 150 mM Fusarium lateritium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
43
-
 CN-
-
 Fusarium lateritium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Fusarium lateritium
300000 400000 gel filtration Fusarium lateritium

Organism

Organism UniProt Comment Textmining
Fusarium lateritium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fusarium lateritium

Reaction

Reaction Comment Organism Reaction ID
formamide = cyanide + H2O reaction proceeds irreversibly in opposite direction Fusarium lateritium
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1109
-
-
 Fusarium lateritium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
HCN + H2O
-
 Fusarium lateritium formamide
-
 ir

Subunits

Subunits Comment Organism
polymer x * 43000, SDS-PAGE Fusarium lateritium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Fusarium lateritium