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Literature summary for 4.2.1.65 extracted from

  • Yanase, H.; Sakai, T.; Tonomura, K.
    Metabolism of nitriles in microorganisms. Part V. Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13 (1983), Agric. Biol. Chem., 47, 473-482.
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Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas sp.

General Stability

General Stability Organism
very unstable, dithiothreitol, GSH and 2-mercaptoethanol protect Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl poor Pseudomonas sp.
Ag+ the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
Cd2+ the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
Cu2+ the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
HgCl2 the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
iodoacetic acid poor Pseudomonas sp.
Mersalyl acid
-
 Pseudomonas sp.
N-ethylmaleimide poor Pseudomonas sp.
NaF poor Pseudomonas sp.
NaNO2 poor Pseudomonas sp.
p-chloromercuribenzoate
-
 Pseudomonas sp.
p-chloromercuriphenyl sulfonic acid
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
83
-
 Beta-cyano-L-alanine
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Pseudomonas sp.
1000000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-Cyano-L-alanine + H2O Pseudomonas sp. cyanide assimilation ?
-
 ?
beta-Cyano-L-alanine + H2O Pseudomonas sp. 13 cyanide assimilation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 13
-
Pseudomonas sp. 13
-
 13
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
54.7
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-Cyano-L-alanine + H2O highly specific for beta-cyano-L-alanine Pseudomonas sp. L-Asparagine
-
 ?
beta-Cyano-L-alanine + H2O highly specific for beta-cyano-L-alanine Pseudomonas sp. 13 L-Asparagine
-
 ?
beta-Cyano-L-alanine + H2O cyanide assimilation Pseudomonas sp. ?
-
 ?
beta-Cyano-L-alanine + H2O cyanide assimilation Pseudomonas sp. 13 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 65
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 up to, 10 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 11
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 in presence of 2-mercaptoethanol Pseudomonas sp.