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Literature summary for 4.2.1.53 extracted from
- Production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from Lysinibacillus fusiformis (2013), Appl. Microbiol. Biotechnol., 97, 8987-8995.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Lysinibacillus fusiformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methanol | 45% residual activity at 8% (v/v) methanol | Lysinibacillus fusiformis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-10-Hydroxystearate | Lysinibacillus fusiformis | - |
Oleate + H2O | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Methanol | 100% activity in the presence of 1-4% (v/v) methanol | Lysinibacillus fusiformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus fusiformis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap column chromatography, and gel filtration | Lysinibacillus fusiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-10-Hydroxystearate | - |
Lysinibacillus fusiformis | Oleate + H2O | - |
? | |
| ricinoleic acid | 90% conversion | Lysinibacillus fusiformis | 10,12-dihydroxystearic acid + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OA hydratase | - |
Lysinibacillus fusiformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Lysinibacillus fusiformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | after 1 h incubation, the enzyme remains stable between 20 and 40°C (100% activity), at 45°C, 50°C, 55°C, 60°C, and 70°C it shows 80%, 70%, 45%, 20% and 0% residual activity, respectively | Lysinibacillus fusiformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Lysinibacillus fusiformis |
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Release 2023.1 (January 2023)
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