Literature summary for 4.2.1.51 extracted from

Van Vleet, J.; Kleeb, A.; Kast, P.; Hilvert, D.; Cleland, W.W.
13C isotope effect on the reaction catalyzed by prephenate dehydratase (2010), Biochim. Biophys. Acta, 1804, 752-754.

Search for more literature for 4.2.1.51

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli KA13, transformed with the plasmid pAKZ11	Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
prephenate	13C isotope effect for conversion of prephenate is 1.0334, the size of this isotope effect suggests that the reaction is concerted. Only residue capable of acting as the general acid needed for removal of the hydroxyl group is threonine-172, which is contained in a conserved TRF motif. More favorable entropy of activation for the enzyme-catalyzed process (25 eu larger than for the acid-catalyzed reaction) may be due to preorganized microenvironment that obviates the need for extensive solvent reorganization, which is consistent with forced planarity of the ring and side chain, which may place the leaving carboxyl and hydroxyl out of plane. Such distortion of the substrate may be a major contributor to catalysis	Methanocaldococcus jannaschii	phenylpyruvate + H2O + CO2	-	?

Synonyms

Synonyms	Comment	Organism
PDT	-	Methanocaldococcus jannaschii
prephenate dehydratase	-	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)