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Literature summary for 4.2.1.50 extracted from

  • Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.
    Purification and characterization of beta-(pyrazol-1-yl)-L-alanine synthase from Citrullus vulgaris (1984), Phytochemistry, 23, 973-977.
No PubMed abstract available

General Stability

General Stability Organism
sensitive to freezing even in 20% glycerol Citrullus lanatus subsp. vulgaris

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine 50 mM almost complete inhibition Citrullus lanatus subsp. vulgaris
potassium cyanide 10 mM almost complete inhibition Citrullus lanatus subsp. vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
O-acetyl-L-serine
-
Citrullus lanatus subsp. vulgaris
74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris
58000
-
gel filtration Citrullus lanatus subsp. vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-Acetyl-L-serine + pyrazole Citrullus lanatus subsp. vulgaris
-
?
-
?

Organism

Organism UniProt Comment Textmining
Citrullus lanatus subsp. vulgaris
-
water melon
-

Purification (Commentary)

Purification (Comment) Organism
-
Citrullus lanatus subsp. vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Citrullus lanatus subsp. vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.355
-
-
Citrullus lanatus subsp. vulgaris

Storage Stability

Storage Stability Organism
0¦C, 1 month Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with L-serine Citrullus lanatus subsp. vulgaris ?
-
?
O-Acetyl-L-serine + 3-amino-1,2,4-triazole 10.6% of activity with pyrazole Citrullus lanatus subsp. vulgaris ?
-
?
O-Acetyl-L-serine + N-hydroxyurea 8.7% of activity with pyrazole Citrullus lanatus subsp. vulgaris ?
-
?
O-Acetyl-L-serine + pyrazole
-
Citrullus lanatus subsp. vulgaris 3-(Pyrazol-1-yl)-L-alanine + acetate
-
?
O-Acetyl-L-serine + pyrazole
-
Citrullus lanatus subsp. vulgaris ?
-
?
O-Succinyl-L-serine + pyrazole 16.4% of activity with O-Acetyl-L-serine Citrullus lanatus subsp. vulgaris ?
-
?
O-Sulfo-L-serine + pyrazole 2.5% of activity with O-Acetyl-L-serine Citrullus lanatus subsp. vulgaris ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.4 O-acetyl-L-serine + pyrazole Citrullus lanatus subsp. vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5 Citrullus lanatus subsp. vulgaris

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 molecule bound to each subunit Citrullus lanatus subsp. vulgaris
pyridoxal 5'-phosphate maximal activation by 40% at 0.25 mM Citrullus lanatus subsp. vulgaris