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Literature summary for 4.2.1.49 extracted from
- Urocanase (Bacillus subtilis) (1971), Methods Enzymol., 17B, 51-55.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Imidazolepropionate | competitive | Bacillus subtilis |  |
| imidazolone propionate | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.077 | - |
Urocanate | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
sucrose density gradient centrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Urocanate + H2O | Bacillus subtilis | - |
? | - |
? | |
| Urocanate + H2O | Klebsiella aerogenes | - |
? | - |
? | |
| Urocanate + H2O | Bacillus subtilis SH-11 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
derived from SB 19 | - |
| Bacillus subtilis SH-11 | - |
derived from SB 19 | - |
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.278 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Urocanate + H2O | - |
Bacillus subtilis | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| Urocanate + H2O | - |
Klebsiella aerogenes | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| Urocanate + H2O | - |
Bacillus subtilis SH-11 | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| Urocanate + H2O | - |
Bacillus subtilis | ? | - |
? | |
| Urocanate + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
| Urocanate + H2O | - |
Bacillus subtilis SH-11 | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8 | - |
Bacillus subtilis |
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