Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Burkholderia cepacia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-alpha-D-mannose | substrate inhibition | Burkholderia cepacia | |
Mg2+ | high concentrations of MgCl2 (25 mM) have an inhibitory effect on enzyme activity | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.024 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, the enzyme inactive in its absence | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
162000 | - |
gel filtration | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose | Burkholderia cepacia | - |
GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? | |
GDP-alpha-D-mannose | Burkholderia cepacia J2315 | - |
GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | B4EFL2 | - |
- |
Burkholderia cepacia J2315 | B4EFL2 | - |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap chelating column chromatography | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose | - |
Burkholderia cepacia | GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? | |
GDP-alpha-D-mannose | - |
Burkholderia cepacia J2315 | GDP-4-dehydro-alpha-D-rhamnose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Burkholderia cepacia |
Synonyms | Comment | Organism |
---|---|---|
bceN | - |
Burkholderia cepacia |
GDP-D-mannose-4,6-dehydratase | - |
Burkholderia cepacia |
GMD | - |
Burkholderia cepacia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | unaffected by NAD+ or NADP+ | Burkholderia cepacia |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.913 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.977 | - |
GDP-alpha-D-mannose | at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein | Burkholderia cepacia |