Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | enhances GMD activity by 2.3fold | Mortierella alpina | |
DTT | enhances GMD activity by 2.3fold | Mortierella alpina |
Cloned (Comment) | Organism |
---|---|
into pET28a+ to construct pLW1534, expressed in Escherichia coli BL21 as His-tagged fusion protein | Mortierella alpina |
recombinant expression of His-tagged GMD in Escherichia coli | Mortierella alpina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, strong inhibition; strong inhibition at 5 mM | Mortierella alpina | |
Cu2+ | 5 mM, strong inhibition; strong inhibition at 5 mM | Mortierella alpina | |
Fe2+ | 5 mM, strong inhibition; strong inhibition at 5 mM | Mortierella alpina | |
additional information | is not affected by Ca2+ and Mn2+ | Mortierella alpina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
GDP-mannose | pH 8.0, 37°C | Mortierella alpina | |
0.77 | - |
GDP-mannose | recombinant enzyme, pH 8.0, 37°C | Mortierella alpina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, activity is significantly enhanced | Mortierella alpina | |
Mg2+ | activates at 5 mM | Mortierella alpina | |
additional information | divalent cations are not required for GMD activity | Mortierella alpina | |
additional information | GMD activity is not affected by Ca2+ at 5 mM | Mortierella alpina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41200 | - |
3 * 45000, recombinant His-tagged GMD, SDS-PAGE, 3 * 41200, about, sequence calculation | Mortierella alpina |
45000 | - |
3 * 45000, SDS-PAGE | Mortierella alpina |
45000 | - |
3 * 45000, recombinant His-tagged GMD, SDS-PAGE, 3 * 41200, about, sequence calculation | Mortierella alpina |
140000 | - |
gel filtration | Mortierella alpina |
140000 | - |
recombinant His-tagged GMD, native PAGE | Mortierella alpina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose | Mortierella alpina | - |
GDP-4-keto-6-deoxy mannose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alpina | D3JU53 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged GMD from Escherichia coli by nickel affinity chromatography | Mortierella alpina |
to near homogeneity by nickel ion affinity chromatography | Mortierella alpina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose | - |
Mortierella alpina | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
GDP-mannose | - |
Mortierella alpina | GDP-4-keto-6-deoxy mannose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 45000, SDS-PAGE | Mortierella alpina |
trimer | 3 * 45000, recombinant His-tagged GMD, SDS-PAGE, 3 * 41200, about, sequence calculation | Mortierella alpina |
Synonyms | Comment | Organism |
---|---|---|
GDP-D-mannose 4,6-dehydratase | - |
Mortierella alpina |
GMD | - |
Mortierella alpina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mortierella alpina |
37 | - |
recombinant enzyme | Mortierella alpina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | 25°C: about 60% of maximal activity, 55°C: about 70% of maximal activity | Mortierella alpina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.96 | - |
GDP-mannose | pH 8.0, 37°C | Mortierella alpina | |
5.96 | - |
GDP-mannose | recombinant enzyme, pH 8.0, 37°C | Mortierella alpina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mortierella alpina |
9 | - |
recombinant enzyme | Mortierella alpina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | pH 5: 45% of maximal activity, pH 11: 75% of maximal activity | Mortierella alpina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mortierella alpina | |
NADP+ | - |
Mortierella alpina |
General Information | Comment | Organism |
---|---|---|
metabolism | GMD catalyzes the first step in the de novo synthesis pathways of GDP-L-fucose, overview | Mortierella alpina |