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Literature summary for 4.2.1.43 extracted from

  • Watanabe, S.; Shimada, N.; Tajima, K.; Kodaki, T.; Makino, K.
    Identification and Characterization of L-Arabonate Dehydratase, L-2-Keto-3-deoxyarabonate Dehydratase, and L-Arabinolactonase Involved in an Alternative Pathway of L-Arabinose Metabolism: Novel Evolution ary Insight into Sugar Metabolism (2006), J. Biol. Chem., 281, 33521-33536.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y are overexpressed in Escherichia coli cells as a His6-tagged enzyme Azospirillum brasilense

Protein Variants

Protein Variants Comment Organism
Q143E inactive mutant enzyme Azospirillum brasilense
Q143N inactive mutant enzyme Azospirillum brasilense
Q143S inactive mutant enzyme Azospirillum brasilense
Q143T inactive mutant enzyme Azospirillum brasilense
Q143Y inactive mutant enzyme Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Azospirillum brasilense
84800
-
 gel filtration Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense Q1JUQ0
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y Azospirillum brasilense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.8
-
-
 Azospirillum brasilense

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Azospirillum brasilense

Synonyms

Synonyms Comment Organism
L-2-keto-3-deoxyarabonate dehydratase
-
 Azospirillum brasilense
L-KDA dehydratase
-
 Azospirillum brasilense