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Literature summary for 4.2.1.39 extracted from
- One-step synthesis of 2-keto-3-deoxy-D-gluconate by biocatalytic dehydration of D-gluconate (2014), J. Biotechnol., 191, 69-77.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | highly economic one-step biocatalytic synthesis procedure of 2-keto-3-deoxy-D-gluconate. D-Gluconate is completely converted tostereochemically pure 2-dehydro-3-deoxy-D-gluconate without side-product formation. The final 2-dehydro-3-deoxy-D-gluconate yield is approximately 90% | Thermoproteus tenax |
| synthesis | highly economic one-step biocatalytic synthesis procedure of 2-oxo-3-deoxy-D-gluconate from D-gluconate using recombinant gluconate dehydratase (GAD) from the hyperthermophilic crenarchaeon Thermoproteus tenax | Thermoproteus tenax |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermoproteus tenax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | deactivates the enzyme | Thermoproteus tenax |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+ | Thermoproteus tenax | |
| Co2+ | the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+ | Thermoproteus tenax | |
| Mg2+ | the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+ | Thermoproteus tenax | |
| Ni2+ | the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+ | Thermoproteus tenax | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 44000, SDS-PAGE | Thermoproteus tenax |
| 44040 | - |
x * 44040, calculated from sequence | Thermoproteus tenax |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoproteus tenax | G4RJQ1 | - |
- |
| Thermoproteus tenax Kra 1 | G4RJQ1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoproteus tenax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate | - |
Thermoproteus tenax | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| D-gluconate | no activity with D-galactopnate and D-xylonate | Thermoproteus tenax | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| D-gluconate | - |
Thermoproteus tenax Kra 1 | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| D-gluconate | no activity with D-galactopnate and D-xylonate | Thermoproteus tenax Kra 1 | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Thermoproteus tenax |
| ? | x * 44040, calculated from sequence | Thermoproteus tenax |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TTX1156 | - |
Thermoproteus tenax |
| TTX1156 | locus name | Thermoproteus tenax |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
48 h, no loss of activity | Thermoproteus tenax |
| 70 | - |
half-life: 7 h | Thermoproteus tenax |
| 80 | - |
half-life: 1 h | Thermoproteus tenax |
| 90 | - |
half-life: 30 min | Thermoproteus tenax |
| 100 | - |
half-life: 3 min | Thermoproteus tenax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermoproteus tenax |
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