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Literature summary for 4.2.1.39 extracted from

  • Kim, S.; Lee, S.B.
    Identification and characterization of the bacterial D-gluconate dehydratase in Achromobacter xylosoxidans (2008), Biotechnol. Bioprocess Eng., 13, 436-444.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
into the pGEM-T easy vector for sequencing Achromobacter xylosoxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
determined by SDS-PAGE Achromobacter xylosoxidans
63410
-
calculated Achromobacter xylosoxidans
270000
-
native enzyme, tetramer Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-gluconate Achromobacter xylosoxidans substrate specificity 100% 2-dehydro-3-deoxy-D-gluconate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
after ammonium sulfate precipitation chromatography on a Phenyl-Sepharose, a Q-Sepharose, a Mono Q, and a HiTrap desalting column is performed Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
nitrogen source urea Achromobacter xylosoxidans
0.011
-
carbon source DL-glutamate Achromobacter xylosoxidans
0.013
-
carbon source DL-alanine Achromobacter xylosoxidans
0.019
-
nitrogen source bacto peptone Achromobacter xylosoxidans
0.025
-
carbon source D-glucose Achromobacter xylosoxidans
0.027
-
carbon source D-galactose Achromobacter xylosoxidans
0.027
-
nitrogen source yeast extract Achromobacter xylosoxidans
0.031
-
nitrogen source (NH4)SO4 Achromobacter xylosoxidans
0.031
-
nitrogen source glucosamine Achromobacter xylosoxidans
0.036
-
nitrogen source H4NO3 Achromobacter xylosoxidans
0.038
-
carbon source D-galactonate Achromobacter xylosoxidans
0.04
-
nitrogen source casamino acid Achromobacter xylosoxidans
0.042
-
carbon source maltose Achromobacter xylosoxidans
0.046
-
carbon source D-arabinose Achromobacter xylosoxidans
0.05
-
nitrogen source NH4Cl Achromobacter xylosoxidans
0.057
-
carbon source sucrose Achromobacter xylosoxidans
0.111
-
nitrogen source malt extract Achromobacter xylosoxidans
0.13
-
carbon source D-gluconate Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabonate substrate specificity 4% Achromobacter xylosoxidans ?
-
?
D-fuconate substrate specificity 18% Achromobacter xylosoxidans ?
-
?
D-galactonate substrate specificity 14% Achromobacter xylosoxidans 2-dehydro-3-deoxy-D-galactonate + H2O
-
?
D-gluconate substrate specificity 100% Achromobacter xylosoxidans 2-dehydro-3-deoxy-D-gluconate + H2O
-
?
D-mannonate substrate specificity 13% Achromobacter xylosoxidans ?
-
?
D-ribonate substrate specificity 11% Achromobacter xylosoxidans ?
-
?
D-xylonate substrate specificity 82% Achromobacter xylosoxidans ?
-
?
DL-isovalerate substrate specificity 11% Achromobacter xylosoxidans ?
-
?
L-arabonate substrate specificity 2% Achromobacter xylosoxidans ?
-
?
L-mannonate substrate specificity 10% Achromobacter xylosoxidans ?
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Achromobacter xylosoxidans

Synonyms

Synonyms Comment Organism
D-gluconate dehydratase
-
Achromobacter xylosoxidans
gluconate dehydratase
-
Achromobacter xylosoxidans
GNAD
-
Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
standard assay condition Achromobacter xylosoxidans
50
-
optimal activity Achromobacter xylosoxidans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
7.5 9
-
Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
standard assay condition Achromobacter xylosoxidans
8.5
-
optimal activity Achromobacter xylosoxidans