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Literature summary for 4.2.1.39 extracted from
- Identification and characterization of Sulfolobus solfataricus D-gluconate dehydratase: a key enzyme in the non-phosphorylated Entner-Doudoroff pathway (2005), Biochem. J., 387, 271-280.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 17% inhibition | Saccharolobus solfataricus |  |
| dithiothreitol | 1 mM, 24% inhibition | Saccharolobus solfataricus | |
| EDTA | - |
Saccharolobus solfataricus | |
| glutathione | 1 mM, 41% inhibition | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
D-gluconate | - |
Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, activates. Presence of one Co2+ per enzyme monomer | Saccharolobus solfataricus | |
| Mg2+ | 1 mM, activates | Saccharolobus solfataricus | |
| Mn2+ | 1 mM, activates | Saccharolobus solfataricus | |
| Ni2+ | 1 mM, activates | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
8 * 44000, SDS-PAGE | Saccharolobus solfataricus |
| 350000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate | Saccharolobus solfataricus | key enzyme in the non-phosphorylated Entner-Doudoroff pathway | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation and dephosphorylation control the activity of D-gluconate dehydratase, loss of activity by dephosphorylation | Saccharolobus solfataricus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.04 | - |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactoheptonate | 1.6% of the activity with D-gluconate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-galactoheptonate + H2O | - |
? | |
| D-galactonate | 2.8% of the activity with D-gluconate. Studies by other groups reveal a catalytic efficiency of 20% for D-galactonate compared to D-gluconate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
| D-gluconate | - |
Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| D-gluconate | key enzyme in the non-phosphorylated Entner-Doudoroff pathway | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 44000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 90 | - |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 95 | 70°C: about 40% of maximal activity, 95°C: about 40% of maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
stable for 2 h | Saccharolobus solfataricus |
| 90 | - |
half-life: 120 min | Saccharolobus solfataricus |
| 100 | - |
half-life: less than 40 min | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 760 | - |
D-gluconate | - |
Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Saccharolobus solfataricus |
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