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Literature summary for 4.2.1.39 extracted from

  • Gottschalk, G.; Bender, R.
    D-gluconate dehydratase from Clostridium pasteurianum (1982), Methods Enzymol., 90, 283-287.
    View publication on PubMed
Search for more literature for 4.2.1.39

Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl compounds e.g. 2-mercaptoethanol or dithiothreitol, activate and inhibit Clostridium pasteurianum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 56% inhibition at 10 mM Clostridium pasteurianum
dithiothreitol 63% inhibition at 10 mM Clostridium pasteurianum
L-cysteine 35% inhibition at 10 mM Clostridium pasteurianum
sulfhydryl compounds
-
 Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
Fe2+ at 2 mM most effective, activates and reactivates after O2 inactivation, probably two distinct sites for activation and reactivation, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 2 * 64000, SDS-PAGE Clostridium pasteurianum
131000
-
 sedimentation equilibrium, gel filtration Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
132
-
-
 Clostridium pasteurianum

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Clostridium pasteurianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 enzyme assay at Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.8
-
 Clostridium pasteurianum