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Literature summary for 4.2.1.35 extracted from
- Purification and properties of citraconase (1968), J. Biol. Chem., 243, 2367-2372.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cys | sulfhydryl compound is required for maximal activity | Pseudomonas sp. |  |
| GSH | sulfhydryl compound is required for maximal activity | Pseudomonas sp. | |
| mercaptoethanol | sulfhydryl compound is required for maximal activity | Pseudomonas sp. | |
| Thioglycollate | sulfhydryl compound is required for maximal activity | Pseudomonas sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH | Pseudomonas sp. | |
| Cu2+ | - |
Pseudomonas sp. | |
| diphosphate | 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH | Pseudomonas sp. | |
| EDTA | 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH | Pseudomonas sp. | |
| Hg2+ | - |
Pseudomonas sp. | |
| iodoacetate | - |
Pseudomonas sp. | |
| PCMB | - |
Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
2-methylmaleate | - |
Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for maximal activity | Pseudomonas sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Methylmaleate + H2O | - |
Pseudomonas sp. | (R)-2-Methylmalate | i.e. L-citramalate | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.2 | pH 6.3: about 30% of maximal activity, pH 8.2: about 55% of maximal activity | Pseudomonas sp. |
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